Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2022 Mar 21;8(1):61-72.
doi: 10.3934/microbiol.2022006. eCollection 2022.

Comparative genome analysis of the first Listeria monocytogenes core genome multi-locus sequence types CT2050 AND CT2051 strains with their close relatives

Ogueri Nwaiwu  1
Affiliations
Review

Comparative genome analysis of the first Listeria monocytogenes core genome multi-locus sequence types CT2050 AND CT2051 strains with their close relatives

Ogueri Nwaiwu. AIMS Microbiol. .

Abstract

Genome sequences of the three strains of L. monocytogenes, which are the first core genome multi-locus sequence types (cgMLST) 2050 and 2051 were reviewed and compared with 21 close relatives and reference genomes. Using a pan-genomic approach to analyse whole genome sequences, it was found that the strains consisted of approximately 2200 shared genes and a much greater pool of genes present as an accessory genome. An unknown transmissible sequence of approximately 91 kb harbouring bacitracin resistance genes found in strain LmNG2 (1/2b) was revealed to be an Inc18 plasmid. The CT2051, strain LmNG3 (1/2a) haboured more unique genes (252 vs 230) than the well-known reference strain LmEGD-e (1/2a). More studies to monitor new strains can help reduce food-borne outbreaks.

Keywords: Listeria monocytogenes; antimicrobial resistance genes; bacitracin genes; genomics; pan-genome; plasmid.

PubMed Disclaimer

Conflict of interest statement

Conflict of interest: The author declares no conflict of interest.

Figures

Figure 1.
Figure 1.. Analysis of L. monocytogenes genomes. Panels include new and unique genes (a), pan genomic pie (b), total and conserved genes(c), and genes distribution (d).
Figure 2.
Figure 2.. Pangenomic matrix (a) and a cluster dendrogram of strains analysed (b).
Figure 3.
Figure 3.. Putative plasmid regions separate from the main genome graph (a) and a BRIG construction for homologues of the ≈90 Kb plasmid sequence (b).
See this image and copyright information in PMC

References

    1. Filipello V, Mughini-Gras L, Gallina S, et al. Attribution of Listeria monocytogenes human infections to food and animal sources in Northern Italy. Food Microbiol. 2020;89:103433. doi: 10.1016/j.fm.2020.103433. - DOI - PubMed
    1. Kallipolitis B, Gahan CGM, Piveteau P. Factors contributing to Listeria monocytogenes transmission and impact on food safety. Curr Opin Food Sci. 2020;36:9–17. doi: 10.1016/j.cofs.2020.09.009. - DOI
    1. Nwaiwu O, Wong L, Lad M, et al. Properties of the extracellular polymeric substance layer from minimally grown planktonic cells of Listeria monocytogenes. Biomolecules. 2021;11:331. doi: 10.3390/biom11020331. - DOI - PMC - PubMed
    1. Nwaiwu O. Molecular serotype and evolutionary lineage of Listeria monocytogenes isolated from different Nigerian food items. Afr J Biotechnol. 2016;15:696–705. doi: 10.5897/AJB2015.15125. - DOI
    1. Nwaiwu O, Moura A, Thouvenot P, et al. Draft genome sequences of Listeria monocytogenes, isolated from fresh leaf vegetables in Owerri City, Nigeria. Genome Announc. 2017;5:e00354–17. doi: 10.1128/genomeA.00354-17. - DOI - PMC - PubMed

LinkOut - more resources