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. 1987 Apr;169(4):1760-2.
doi: 10.1128/jb.169.4.1760-1762.1987.

Cloning and expression in Escherichia coli of a cellulase gene from Ruminococcus flavefaciens

M E Barros, J A Thomson

Cloning and expression in Escherichia coli of a cellulase gene from Ruminococcus flavefaciens

M E Barros et al. J Bacteriol. 1987 Apr.

Abstract

An endoglucanase gene of Ruminococcus flavefaciens FD1 was cloned on the vector pEcoR251 to form the recombinant plasmid pMEB200. The cloned endoglucanase gene showed carboxymethylcellulase enzyme activity but no degradation of Avicel (FMC Corp., Philadelphia, Pa.) or filter paper. Carboxymethylcellulase activity was found during the late-exponential-growth phase and accumulated in the periplasmic fraction. Enzyme production was not subject to catabolite repression by glucose.

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