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. 2022 Apr 13:9:863814.
doi: 10.3389/fvets.2022.863814. eCollection 2022.

Detection of Phenuiviridae, Chuviridae Members, and a Novel Quaranjavirus in Hard Ticks From Danube Delta

Bianca Elena Bratuleanu  1   2 , Sarah Temmam  1   3 , Sandie Munier  4 , Delphine Chrétien  1   3 , Thomas Bigot  1 , Sylvie van der Werf  4   5 , Gheorghe Savuta  2 , Marc Eloit  1   3   6
Affiliations

Detection of Phenuiviridae, Chuviridae Members, and a Novel Quaranjavirus in Hard Ticks From Danube Delta

Bianca Elena Bratuleanu et al. Front Vet Sci. .

Abstract

Ticks are involved in the transmission of various pathogens and several tick-borne diseases cause significant problems for the health of humans and livestock. The members of the Quaranjavirus genus are mainly associated with argas ticks but recent studies demonstrated the presence of novel quaranjaviruses-like in ixodid ticks. In 2020, 169 Rhipicephalus sanguineus ticks were collected in Southern Romania from small ruminants and analyzed by high-throughput transcriptome sequencing. Among the viral families that infect Romanian ticks, we have identified sequences from Phenuiviridae (Brown dog tick phlebovirus 1 [BDTPV1] and Brown dog tick phlebovirus 2 [BDTPV2]) and Chuviridae families (Cataloi mivirus [CTMV]), and numerous sequences from a new quaranjavirus-like, tentatively named Cataloi tick quaranjavirus (CTQV). Phylogenetic analyses performed on the five segments show that CTQV is phylogenetically positioned within a clade that encompasses Ixodidae-borne viruses associated with iguanas, small ruminants, seabirds, and penguins distributed across different geographical areas. Furthermore, CTQV is positioned differently depending on the segment considered. This is the first report on the detection of a quaranjavirus-like in Eastern Europe. Further investigations are needed to discern its infectivity and pathogenicity against vertebrates.

Keywords: Eastern Europe; next generation sequencing; quaranjaviruses; small ruminant; ticks.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Phylogenetic relationship of nucleoprotein and RNA-dependent RNA polymerase of BDTPV1 and BDTPV2 identified in Romanian Rhipicephalus ticks with other viruses among the Phlebovirus genus.
Figure 2
Figure 2
Phylogenetic relationship of RNA-dependent RNA polymerase of Cataloi mivirus (CTMV) identified in Romanian Rhipicephalus ticks with other viruses among the Chuviridae family.
Figure 3
Figure 3
Phylogenetic analysis of Cataloi tick quaranjavirus (CTQV), PB1, PB2, PA, NP, and HA proteins with other viruses in the Orthomyxoviridae family. In light blue: Ixodidae-associated quaranjaviruses; in dark blue: Argasidae-associated quaranjaviruses, in pink: vertebrates associated quaranjaviruses.
See this image and copyright information in PMC

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