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. 2022 Jan 4:13:100206.
doi: 10.1016/j.fochx.2022.100206. eCollection 2022 Mar 30.

Effects of Ilisha elongata proteins on proliferation and adhesion of Lactobacillus plantarum

Guoyan Liu  1 , Meng Chu  1 , Piao Xu  2 , Shiying Nie  2 , Xin Xu  1 , Jiaoyan Ren  2
Affiliations

Effects of Ilisha elongata proteins on proliferation and adhesion of Lactobacillus plantarum

Guoyan Liu et al. Food Chem X. .

Abstract

The effects of aquatic proteins on the proliferation and adhesion of intestinal probiotic bacteria were investigated by in vitro fermentation and mouse in vitrointestinal tissue models. Compared with the control group, the Illisha elongata protein reduced the growth time of Lactobacillus plantarum (LP45) by 34.25% and increased the total number of colonies by 6.61%. The Ilisha elongata salt-solubale protein performed better than water-soluble protein in vitro proliferation of LP45. Ilisha elongata salt-soluble protein significantly increased the number of viable bacteria adhering to intestinal, and caused changes in the amount of polysaccharides, proteins and biofilms in the intestinal tissue model. These results indicate that the Ilisha elongata protein is beneficial to the proliferation and adhesion of probiotics in the intestinal, and can be used as an active protein beneficial to intestinal health.

Keywords: Adhesion; Ilisha elongata proteins; Lactobacillus plantarum; Proliferation.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
Schematic diagram of cultivating 3 kinds of probiotics with 8 kinds of aquatic proteins(A);Growth curves of M8, LP45, BBi32 (B, C,D); Growth generation times of M8, LP45, BBi32 (E, F,G); The total number of colonies of M8, LP45, BBi32 (H, I,J). *p < 0.05, compared with the Pd-MRS group.
Fig. 2
Fig. 2
The total number of colonies (A);Percentage change compared to control (B).***p < 0.005.
Fig. 3
Fig. 3
Growth curve of LP45 (A); Growth time of LP45 (B); Total number of colonies of LP45 (C). **p < 0.01, compared with the MRS group;***p < 0.005, compared with the MRS group;****p < 0.001, compared with the MRS group.
Fig. 4
Fig. 4
Schematic diagram of the mouse ex vivo intestinal tissue adhesion model(A)The number of viable bacteria of LP45 adhering in the intestine (B); Scanning electron micrograph of LP45 adhering in the intestine of MRS experimental group (C-G); Scanning electron micrograph of LP45 adhering in the intestine of MRS + IeP experimental group (H-M). *p < 0.05.
Fig. 5
Fig. 5
Changes in sugar content in each intestinal segment (A); changes in protein content in each intestinal segment (B); changes in biofilm biomass in each intestinal segment (C);Protein molecular weight distribution of each intestinal segment (D); 1–5: protein molecular weight distribution of duodenum, jejunum, ileum, cecum, and colon in control group, respectively. 6–10: protein molecular weight distribution of duodenum, jejunum, ileum, cecum, and colon in MRS group, respectively. 11–15: protein molecular weight distribution of duodenum, jejunum, ileum, cecum, and colon in MRS + IeP group, respectively distribution. 16: protein molecular weight distribution of MRS medium. 17: protein molecular weight distribution of Ilisha elongata protein. 18: protein molecular weight distribution of LP45. 19: protein molecular weight distribution of Ilisha elongata protein culture LP45. control-blank intestinal segment control, IeP-Ilisha elongata protein.
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