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. 2022 Apr;172(6):729-733.
doi: 10.1007/s10517-022-05466-7. Epub 2022 May 2.

Construction and Characterization of HIV-1 env-Pseudoviruses of the Recombinant Form CRF63_02A and Subtype A6

N B Rudometova  1 , N S Shcherbakova  2 , D N Shcherbakov  2 , O S Taranov  2 , B N Zaitsev  2 , L I Karpenko  2
Affiliations

Construction and Characterization of HIV-1 env-Pseudoviruses of the Recombinant Form CRF63_02A and Subtype A6

N B Rudometova et al. Bull Exp Biol Med. 2022 Apr.

Abstract

HIV-1 env-pseudoviruses are a useful tool in the search for antiviral drugs (entry inhibitors) and evaluation of the efficacy of HIV-1 vaccines. Given the high genetic variability of HIV-1, it is necessary to regularly update the panels of pseudoviruses in accordance with the emergence of new strains. Based on genetic variants of HIV-1 circulating in the regions of the Siberian Federal District, 13 HIV-1 env-pseudoviruses of recombinant form CRF63_02A and subtype A6 were obtained. Most pseudoviruses have been shown to be sensitive to neutralization by bnAbs VRC01, PGT126, and 10E8, moderately sensitive to bnAbs PG9 and 4E10, and resistant to bnAbs 2G12, PG16, and 2F5. All obtained variants of pseudoviruses are CCR5-tropic.

Keywords: A6; CRF63_02A; broadly neutralizing antibodies (bnAbs); env-pseudoviruses; virus neutralization assay.

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References

    1. Baryshev PB, Bogachev VV, Gashnikova NM. HIV-1 genetic diversity in Russia: CRF63_02A1, a new HIV type 1 genetic variant spreading in Siberia. AIDS Res. Hum. Retroviruses. 2014;30(6):592-597. https://doi.org/10.1089/aid.2013.019 - DOI - PubMed - PMC
    1. Chong H, Hong K, Zhang C, Nie J, Song A, Kong W, Wang Y. Genetic and neutralization properties of HIV-1 env clones from subtype B/BC/AE infections in China. J. Acquir. Immune Defic. Syndr. 2008;47(5):535-543. https://doi.org/10.1097/QAI.0b013e3181663967 - DOI - PubMed
    1. Hraber P, Rademeyer C, Williamson C, Seaman MS, Gottardo R, Tang H, Greene K, Gao H, LaBranche C, Mascola JR, Morris L, Montefiori DC, Korber B. Panels of HIV-1 subtype C Env reference strains for standardized neutralization assessments. J. Virol. 2017;91(19):e00991-17. https://doi.org/10.1128/JVI.00991-17 - DOI - PubMed - PMC
    1. Lebedev A, Lebedeva N, Moskaleychik F, Pronin A, Kazennova E, Bobkova M. Human Immunodeficiency Virus-1 diversity in the Moscow Region, Russia: phylodynamics of the most common subtypes. Front. Microbiol. 2019;10:320. https://doi.org/10.3389/fmicb.2019.00320 - DOI - PubMed - PMC
    1. Li M, Gao F, Mascola J.R, Stamatatos L, Polonis VR, Koutsoukos M, Voss G, Goepfert P, Gilbert P, Greene KM, Bilska M, Kothe DL, Salazar-Gonzalez JF, Wei X, Decker JM, Hahn BH, Montefiori DC. Human immunodeficiency virus type 1 env clones from acute and early subtype B infections for standardized assessments of vaccine-elicited neutralizing antibodies. J. Virol. 2005;79(16):10108-10125. https://doi.org/10.1128/JVI.79.16.10108-10125.2005 - DOI - PubMed - PMC

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