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. 2022 Aug;1514(1):93-103.
doi: 10.1111/nyas.14782. Epub 2022 May 4.

Cross-talk of four types of RNA modification writers defines the immune microenvironment in severe asthma

Qibin Lin  1 , Haiyang Ni  1 , Zhishui Zheng  1 , Jieying Zhong  1 , Hanxiang Nie  1
Affiliations

Cross-talk of four types of RNA modification writers defines the immune microenvironment in severe asthma

Qibin Lin et al. Ann N Y Acad Sci. 2022 Aug.

Abstract

Adenine modifications, including m6 A, m1 A, APA, and A-to-I modifications, are the most impactful RNA modifications. These modifications are primarily produced by enzymes called writers. The main purpose of this study was to explore the cross-talk and potential roles of these writers in severe asthma. We found 13 RNA writers potentially related to severe asthma and three RNA modification patterns. Cluster 3 showed predominant neutrophil infiltration and C-type lectin receptor signaling; cluster 1 showed predominant innate immune cell infiltration and ubiquitin-proteasome system activation; and cluster 2 did not show obvious immune infiltration characteristics. We found that RNA modification writers modified immune cell-related genes and led to both accumulation of different immune cells in the airways and activation of a series of biological processes, which ultimately leads to severe asthma. TRMT6, WTAP, and TRMT6A were included in a random forest model as predictors. Cromoglicic acid, thioperamide, and fluvastatin were potential drugs for clusters 1, 2, and 3, respectively. We found that cross-talk of RNA modifications is significant in severe asthma, which provides insight into severe asthma pathogenesis and possible treatment avenues.

Keywords: RNA modification pattern; RNA modification writers; epigenetics; immune microenvironment; severe asthma; treatment.

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References

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