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. 2022 Jul 11;28(39):e202201180.
doi: 10.1002/chem.202201180. Epub 2022 May 25.

HPLC-Based Automated Synthesis of Glycans in Solution

Samira Escopy  1   2 , Yashapal Singh  1 , Keith J Stine  1 , Alexei V Demchenko  1   2
Affiliations

HPLC-Based Automated Synthesis of Glycans in Solution

Samira Escopy et al. Chemistry. .

Abstract

As the 21st century unfolds with rapid changes, new challenges in research and development emerge. These new challenges prompted us to repurpose our HPLC-A platform that was previously used in solid phase glycan synthesis to a solution phase batch synthesis described herein. The modular character of HPLC allows for implementing new attachments. To enable sequential synthesis of multiple oligosaccharides with the single press of a button, we supplemented our system with a four-way split valve and an automated fraction collector. This enabled the operator to load all reagents and all reactants in the autosampler, press the button to start the repetitive automation sequence, leave the lab, and upon return find products of multiple reactions ready for purification, analysis, and subsequent application.

Keywords: automation; glycans; glycosylation; oligosaccharides; synthesis.

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Conflict of interest statement

Conflict of Interest

The authors declare no conflict of interest.

Figures

Scheme 1.
Scheme 1.
Generation C HPLC–A on solid phase (A) and HPLC–A in solution (B).
Scheme 2.
Scheme 2.
HPLC–A synthesis of multiple disaccharides 11–14 with a single press of a button.resolution is low, providing below a higher resolution image
Scheme 3.
Scheme 3.
HPLC–A synthesis of di- and trisaccharides 12, 43–47 by different methods with a single press of a button.resolution is low, providing below a higher resolution image
Scheme 4.
Scheme 4.
HPLC–A synthesis of protected HMOs LNTri II and ß3’-GL with a single press of a button.
See this image and copyright information in PMC

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