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Review
. 2022 Apr 14:14:100259.
doi: 10.1016/j.mtbio.2022.100259. eCollection 2022 Mar.

Microphysiological stem cell models of the human heart

Ulgu Arslan  1 , Alessia Moruzzi  2   3 , Joanna Nowacka  4 , Christine L Mummery  1 , Dominik Eckardt  4 , Peter Loskill  2   3   5 , Valeria V Orlova  1
Affiliations
Review

Microphysiological stem cell models of the human heart

Ulgu Arslan et al. Mater Today Bio. .

Abstract

Models of heart disease and drug responses are increasingly based on human pluripotent stem cells (hPSCs) since their ability to capture human heart (dys-)function is often better than animal models. Simple monolayer cultures of hPSC-derived cardiomyocytes, however, have shortcomings. Some of these can be overcome using more complex, multi cell-type models in 3D. Here we review modalities that address this, describe efforts to tailor readouts and sensors for monitoring tissue- and cell physiology (exogenously and in situ) and discuss perspectives for implementation in industry and academia.

Keywords: Cardiac microtissue; Cardiomyocyte maturation; Engineered heart tissue; Functional readout; Heart-on-chip; Human induced pluripotent stem cells; Multicellular cell diseases and drug efficacy platform; Structural readout.

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Conflict of interest statement

J.N and D.E. are employees of Miltenyi Biotec B.V. & Co. KG.

Figures

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Graphical abstract
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