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. 2019 Jul 1;9(35):20432-20438.
doi: 10.1039/c9ra03643a. eCollection 2019 Jun 25.

Pullulan nanofibers containing the antimicrobial palindromic peptide LfcinB (21-25)Pal obtained via electrospinning

Julieth Tatiana Román  1 , Carlos Alberto Fuenmayor  2 , Carlos Mario Zuluaga Dominguez  3 , Dianney Clavijo-Grimaldo  4 , Martha Acosta  5 , Javier Eduardo García-Castañeda  1 , Ricardo Fierro-Medina  1 , Zuly Jenny Rivera-Monroy  1
Affiliations

Pullulan nanofibers containing the antimicrobial palindromic peptide LfcinB (21-25)Pal obtained via electrospinning

Julieth Tatiana Román et al. RSC Adv. .

Abstract

Electrospinning technology is useful for making ultrafine drug-eluting fibers for the clinical treatment of wounds. We show the incorporation of an antimicrobial LfcinB-derived peptide into Pullulan nanofibers. The palindromic peptide LfcinB (21-25)Pal: RWQWRWQWR was synthesized, purified, and characterized by means of the RP-HPLC and MALDI-TOF MS methods. The peptide's antibacterial activity against the E. coli ATCC 25922 strain was evaluated, and the peptide LfcinB (20-25)Pal exhibited significant antibacterial activity. Nanofibers were obtained by electrospinning a Pullulan or Pullulan-LfcinB (21-25)Pal solution. The obtained nanofibers were characterized via microscopy (AFM and SEM) and RP-HPLC chromatography. The peptide incorporation efficiency was 31%. The Pullulan-LfcinB (21-25)Pal nanofibers were soluble in water, and the peptide was liberated immediately. The Pullulan-LfcinB (21-25)Pal nanofibers exhibited the same antibacterial activity against E. coli strain as the free peptide LfcinB (21-25)Pal. The results suggest that Pullulan-LfcinB (21-25)Pal nanofibers could be considered for designing and developing antibacterial wound dressings.

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Conflict of interest statement

There are no conflicts to declare.

Figures

Fig. 1
Fig. 1. Chromatographic characterization of the peptide and membranes. Peptide LfcinB (21–25)Pal (A) crude, (B) pure; (C) Pullulan-LfcinB (21–25)Pal membrane and (D) Pullulan membrane (control).
Fig. 2
Fig. 2. Membrane characterization. (A) Pullulan-LfcinB (21–25)Pal membrane photography. Membranes were observed under UV radiation at 254 nm: (B) Pullulan-LfcinB (21–25)Pal, and (C) Pullulan membranes.
Fig. 3
Fig. 3. AFM membrane. (A) Pullulan-LfcinB (21–25)Pal and (B) Pullulan membranes. (Right) membrane 3D projection; (left) membrane topographic profile.
Fig. 4
Fig. 4. Nanofiber characterization via SEM. (A)–(C) Pullulan-LfcinB (21–25)Pal nanofibers. (D)–(F) Pullulan nanofibers.
Fig. 5
Fig. 5. Frequency histogram of nanofiber diameter distribution. (A) Pullulan nanofibers; (B) Pullulan-LfcinB (21–25)Pal nanofibers. The ANOVA indicates that there is a significant difference between the diameter of the Pullulan nanofibers and that of the Pullulan-LfcinB (21–25)Pal nanofibers, (n = 100).
Fig. 6
Fig. 6. Membrane antibacterial activity. Determination of MBC of Pullulan-LfcinB (21–25)Pal nanofibers and the peptide LfcinB (21–25)Pal (control). Bacterial growth inhibition of the E. coli strain treated with (A) peptide LfcinB (21–25)Pal and (B) Pullulan-LfcinB (21–25)Pal nanofibers. (n = 2).
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