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. 2019 Feb 21;9(11):6254-6266.
doi: 10.1039/c8ra10573a. eCollection 2019 Feb 18.

Surface hemocompatible modification of polysulfone membrane via covalently grafting acrylic acid and sulfonated hydroxypropyl chitosan

Ming-Ming Tu  1 , Jing-Jie Xu  1 , Yun-Ren Qiu  1
Affiliations

Surface hemocompatible modification of polysulfone membrane via covalently grafting acrylic acid and sulfonated hydroxypropyl chitosan

Ming-Ming Tu et al. RSC Adv. .

Abstract

In this study, acrylic acid (AA) and sulfonated hydroxypropyl chitosan (SHPCS) were covalently grafted on the PSf membrane surface to improve its hemocompatibility. First, the modified AA-PSf membrane was obtained through the Friedel-Craft reaction between acrylic acid and the PSf membrane surface. Then, the modified SHPCS-AA-PSf membrane was prepared by grafting SHPCS onto the AA-PSf membrane surface via the dehydration acylation of the carboxyl group of the AA-PSf membrane with the amino group of SHPCS. ATR-FTIR and XPS measurements confirmed that the -COOH group and SHPCS were successfully grafted onto the surface of the PSf membrane. The modified PSf membranes showed suppressed platelet adhesion and lower protein adsorption (161 μg cm-2) compared with the pristine PSf membrane (341 μg cm-2). Hemocompatibility testing showed that modified membrane materials had a prolonged clotting time, plasma recalcification time (PRT), activated partial thromboplastin time (APTT), thrombin time (TT), and prothrombin time (PT). All of these results indicated that the surface modification of the PSf membrane with acrylic acid and SHPCS had good hemocompatibility and anticoagulant property.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Fig. 1
Fig. 1. The steps of the modification of the PSf membrane.
Fig. 2
Fig. 2. The grafting density of acrylic acid at different reaction times (a); the grafting density of SHPCS and the SHPCS-AA-PSf water contact angle at different reaction times (b).
Fig. 3
Fig. 3. ATR-FTIR spectra of PSf, AA-PSf and SHPCS-AA-PSf membranes.
Fig. 4
Fig. 4. XPS spectra of PSf, AA-PSf, and SHPCS-AA-PSf membranes (a); spectra analysis of the PSf membrane (b), AA-PSf membrane (c) and SHPCS-AA-PSf membrane (d).
Fig. 5
Fig. 5. SEM images of PSf, AA-PSf and SHPCS-AA-PSf membrane surfaces (a); SEM photographs of PSf, AA-PSf and SHPCS-AA-PSf membrane cross-sections (b).
Fig. 6
Fig. 6. Surface contact angles of the PSf membrane, AA-PSf membrane and SHPCS-AA-PSf membrane.
Fig. 7
Fig. 7. The standard curve of BSA absorbance (a); the adsorption of BSA on PSf, AA-PSf and SHPCS-AA-PSf membranes (b).
Fig. 8
Fig. 8. The SEM image of platelets adsorbed onto PSf, AA-PSf and SHPCS-AA-PSf membranes. Magnification: 1000× (a); magnification: 5000× (b).
Fig. 9
Fig. 9. The plasma recalcification time of PSf, AA-PSf and SHPCS-AA-PSf membranes (a); the hemolysis rate of PSf, AA-PSf and SHPCS-AA-PSf membranes (b); the APTT, PT and TT of PSf, AA-PSf and SHPCS-AA-PSf membranes (c).
Fig. 10
Fig. 10. Images of P. aeruginosa under different culture conditions (a); effects of blank, PSf, AA-PSf and SHPCS-AA-PSf membrane on the survival number of P. aeruginosa (b).
See this image and copyright information in PMC

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