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. 2020 Oct 23;10(64):38818-38830.
doi: 10.1039/d0ra05933a. eCollection 2020 Oct 21.

Ionic magnetic core-shell nanoparticles for DNA extraction

Tammar Hussein Ali  1   2 , Amar Mousa Mandal  3 , Thorsten Heidelberg  1 , Rusnah Syahila Duali Hussen  1 , Ean Wai Goh  1
Affiliations

Ionic magnetic core-shell nanoparticles for DNA extraction

Tammar Hussein Ali et al. RSC Adv. .

Abstract

Magnetic nanoparticles with specific surface features are interesting materials for biomedical applications. The combination of molecular interactions on small particles with macroscopic cohesion forces offers unique opportunities. This work reports the synthesis of magnetic core-shell nanoparticles with alkylimidazolium coated surface for effective DNA extraction. A magnetic Fe2O3 core was coated with a silica shell and functionalized with an organic halide. This enabled a surface coating with organic cations to mediate effective molecular interactions with polyanionic DNA. The large surface area of the ∼20 nm small particles with a magnetization of 25 emu g-1 enabled high DNA particle loading of 1/30 m% with easy isolation based on an external magnetic field. Moreover, the coating of the particles stabilized DNA against ultrasound initiated fragmentation.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig. 1
Fig. 1. Synthetic pathways for the synthesis of ionic magnetic nanoparticles: (A) synthesis of core–shell magnetic nanoparticles, (B) grafting and immobilization of magnetic nanoparticles.
Fig. 2
Fig. 2. XRD patterns of magnetic nanoparticles.
Fig. 3
Fig. 3. FESEM images of NpFeSiImR.
Fig. 4
Fig. 4. (A) and (B) HRTEM image of NpFeSiImR nanoparticles with lattice fringes display. (C) The partical size of NpFeSiImR nanoparticles.
Fig. 5
Fig. 5. EDX analysis of core–shell nanoparticles NpFeSi.
Fig. 6
Fig. 6. EDX analysis of surface functionalized nanoparticles NpFeSiCl and NpFeSiImR.
Fig. 7
Fig. 7. ATR FT-IR spectra of NpFeSiPrCl and NpFeSiImR.
Fig. 8
Fig. 8. Super-paramagnetic hysteresis loops for NpFeSiCl and NpFeSiImR nanoparticles.
Fig. 9
Fig. 9. Shows electrophoretic DNA profiles for recovering process.
Fig. 10
Fig. 10. Gel retardation assay of ultrasound-exposed DNA after increasing exposure times [s]. (A) Scrambled DNA; (B) DNA–MNPs complexes.
Fig. 11
Fig. 11. Gel retardation assay of DNA–NPs complex with different alkyl chain.
Fig. 12
Fig. 12. Gel retardation assay of DNA–NPs complex with NpFeSiImC12, NpFeSiCl, and NpFeSi.
Fig. 13
Fig. 13. Complexation test of magnetic Nps and DNA in water. Each DNA–Nps complex was prepared at a weight ratio (10, 20, 30, 40 and 50) of MNPs to DNA.
Fig. 14
Fig. 14. Gel retardation assay of extraction of DNA from blood sample (DNA–NPs complex with NpFeSiImR.).
See this image and copyright information in PMC

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