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. 2019 Feb 25;9(12):6548-6555.
doi: 10.1039/c8ra09827a. eCollection 2019 Feb 22.

Phospholipase D encapsulated into metal-surfactant nanocapsules for enhancing biocatalysis in a two-phase system

Hui Li  1   2 , Yang Pang  1   2 , Xin Wang  1   2 , Xun Cao  1   2 , Xun He  1   2 , Kequan Chen  1   2 , Ganlu Li  1   2 , Pingkai Ouyang  1   2 , Weiming Tan  3
Affiliations

Phospholipase D encapsulated into metal-surfactant nanocapsules for enhancing biocatalysis in a two-phase system

Hui Li et al. RSC Adv. .

Abstract

Methods for enhancing enzyme activities in two-phase systems are getting more attention. Phospholipase D (PLD) was successfully encapsulated into metal-surfactant nanocapsules (MSNCs) using a one-pot self-assembly technique in an aqueous solution. The highest yield for the production of high-value phosphatidylserine (PS) from low-value phosphatidylcholine (PC) in the two-phase system was achieved by encapsulating PLD into MSNCs formed from Ca2+ which gave an enzyme activity that was 133.6% of that of free PLD. The PLD@MSNC transformed the two-phase system into an emulsion phase system and improved the organic solvent tolerance, pH and thermal stabilities as well as the storage stability and reusability of the enzyme. Under optimal conditions, PLD@MSNC generated 91.9% PS over 8 h in the two-phase system, while free PLD generated only 77.5%.

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Conflict of interest statement

There are no conflicts to declare.

Figures

Fig. 1
Fig. 1. Effects of metal ions (Co2+, Ca2+, Mg2+, Ni2+, and Zn2+) on the activities of free PLD and PLD@MSNC.
Fig. 2
Fig. 2. (a) Scanning electron microscopy (SEM) image of MSNCs. (b) SEM image of PLD@MSNC. (c) SEM image of PLD@MSNC in the two-phase reaction system.
Fig. 3
Fig. 3. (a) Fourier-transform infrared spectroscopy (FTIR) analysis of free PLD, MSNCs, and PLD@MSNC in the aqueous phase, and PLD@MSNC in the two-phase reaction system. (b) Thermogravimetric analysis (TGA) curves of MSNCs and PLD@MSNC in a nitrogen atmosphere. (c) N2 adsorption–desorption isotherms of MSNCs and PLD@MSNC.
Fig. 4
Fig. 4. (a) The relative activities of free PLD and PLD@MSNC in different solvents. (b) Effect of reaction temperature on the yields of PS obtained with free PLD and PLD@MSNC. (c) Effect of reaction pH on the yields of PS obtained with free PLD and PLD@MSNC.
Fig. 5
Fig. 5. Time-dependence of biosynthesis and accumulation of PS using different concentrations of PC with free PLD or PLD@MSNC in the two-phase system and the aqueous system.
Fig. 6
Fig. 6. Storage stability of free PLD and PLD@MSNC at 4 °C and at room temperature over 28 consecutive days.
Fig. 7
Fig. 7. (a) Reusability of PLD@MSNC over eight successive cycles of biosynthesis of PS from PC in the two-phase system. (b) PLD loss from PLD@MSNC over eight successive cycles in the two-phase system.
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