doi: 10.1007/978-1-4684-5251-8_16.
Sequence of the M1-2 region of killer virus double-stranded RNA
- PMID: 3551914
- DOI: 10.1007/978-1-4684-5251-8_16
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Sequence of the M1-2 region of killer virus double-stranded RNA
Basic Life Sci.
1986.
Abstract
A full-length complementary DNA (cDNA) copy of the M1-2 region of the double-stranded genome of the yeast killer virus was synthesized by reverse transcription, utilizing the m in vitro transcript as template and synthetic primers for both strands. The sequence lacks any long open reading frames (ORFs). The internal portion of the M1-2 region includes the sequence that is linked to the subterminal 229 bases of the M1-1 homologous region in the S3 defective-interfering mutant of killer virus double-stranded RNA (dsRNA). Thus, the probable site at which the deletion occurred in S3 has been identified.
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