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. 1986:40:203-13.
doi: 10.1007/978-1-4684-5251-8_16.

Sequence of the M1-2 region of killer virus double-stranded RNA

D E Georgopoulos, E M Hannig, M J Leibowitz

Sequence of the M1-2 region of killer virus double-stranded RNA

D E Georgopoulos et al. Basic Life Sci. 1986.

Abstract

A full-length complementary DNA (cDNA) copy of the M1-2 region of the double-stranded genome of the yeast killer virus was synthesized by reverse transcription, utilizing the m in vitro transcript as template and synthetic primers for both strands. The sequence lacks any long open reading frames (ORFs). The internal portion of the M1-2 region includes the sequence that is linked to the subterminal 229 bases of the M1-1 homologous region in the S3 defective-interfering mutant of killer virus double-stranded RNA (dsRNA). Thus, the probable site at which the deletion occurred in S3 has been identified.

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