Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 1986 Dec;5(6):252-7.
doi: 10.1002/bies.950050605.

Retrovirus vectors and their uses in molecular biology

Review

Retrovirus vectors and their uses in molecular biology

E Gilboa. Bioessays. 1986 Dec.

Abstract

Retroviral vectors utilize the biochemical processes unique to retroviruses, to transfer genes with high efficiency into a wide variety of cell types in tissue culture and in living animals. With such vectors, the effect of newly introduced genes and the mechanism of gene expression can be studied in cell types so far refractory to other methods of transfer.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Wigler, M. , Silverstein, S. , Lee, L. , Pellicer, A. , Chen, V. & Axel, R. (1977). Transfer of purified Herpes virus thymidine kinase gene to cultured mouse cells. Cell 11, 223–232. - PubMed
    1. Schaffner, W. (1980). Direct transfer of cloned genes from bacteria to mammalian cells. Proc. Natl Acad. Sci. USA. 77, 2163–2167. - PMC - PubMed
    1. Potter, H. , Weir, L. & Leder, P. (1984). Enhancer‐dependent expression of human kappa immunoglobulin genes introduced into mouse pre‐B lymphocytes by electroporation. Proc. Natl Acad. Sci. USA 81, 7161–7165. - PMC - PubMed
    1. Mann, R. , Mulligan, R. C. & Baltimore, D. (1983). Construction of a retrovirus packaging mutant and its use to produce helper‐free defective retrovirus. Cell 33, 153–159. - PubMed
    1. Temin, H. M. (1986). Retrovirus vectors for gene transfer; efficient integration into and expression of exogenous DNA in vertebrate cell genomes In Gene Transfer (ed. Kucherlapati R.). Plenum Press, New York.

LinkOut - more resources