Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Feb 6;9(8):4616-4620.
doi: 10.1039/c8ra10164g. eCollection 2019 Jan 30.

Praseodymium promotes B-Z transition in self-assembled DNA nanostructures

Madhabi M Bhanjadeo  1   2 , Umakanta Subudhi  1   2
Affiliations

Praseodymium promotes B-Z transition in self-assembled DNA nanostructures

Madhabi M Bhanjadeo et al. RSC Adv. .

Abstract

Millimolar concentrations of PrCl3 can induce sequence-specific B-Z transition in various-self-assembled branched DNA (bDNA) nanostructures. Competitive dye binding and thermal kinetics suggest that the phosphate backbone and grooves of bDNA are wrapped with Pr3+ for stabilizing the Z-bDNA. Application of EDTA can convert Z-DNA back to the B-form.

PubMed Disclaimer

Conflict of interest statement

There are no conflicts to declare.

Figures

Fig. 1
Fig. 1. Schematic presentation of Pr-induced B–Z transition in bDNA structure and reversal transition by EDTA.
Fig. 2
Fig. 2. (a–d) Circular dichroism spectral changes of bDNA US-17, US-18, US-23 and US-24 induced by 2.5, 5.0, 7.5 and 10 mM PrCl3.
Fig. 3
Fig. 3. (a and b) Circular dichroism spectra of B-bDNA US-23 with MG followed by PrCl3 and (b) CD spectra of Z-bDNA US-23 with MG.
Fig. 4
Fig. 4. (a) CD spectra of B-bDNA US-23 with EtBr followed by PrCl3, (b) CD spectra of Z-bDNA US-23 with EtBr, (c) CD spectra of B-bDNA US-23 with EDTA followed by PrCl3 and (d) CD spectra of Z-bDNA US-23 with EDTA.
See this image and copyright information in PMC

References

    1. Seeman N. C. Annu. Rev. Biochem. 2010;79:65. doi: 10.1146/annurev-biochem-060308-102244. - DOI - PMC - PubMed
    2. Nayak A. K. Subudhi U. RSC Adv. 2014;4:54506. doi: 10.1039/C4RA08873E. - DOI
    3. Saha S. Prakash V. Halder S. Chakraborty K. Krishnan Y. Nat. Nanotechnol. 2015;10:645. doi: 10.1038/nnano.2015.130. - DOI - PubMed
    4. Halley P. D. Lucas C. R. McWilliams E. M. Webber M. J. Patton R. A. Kural C. Lucas D. M. Byrd J. C. Castro C. E. Small. 2016;12:308. doi: 10.1002/smll.201502118. - DOI - PMC - PubMed
    5. Nahar S. Nayak A. K. Ghosh A. Subudhi U. Maiti S. Nanoscale. 2018;10:195. doi: 10.1039/C7NR06601E. - DOI - PubMed
    1. Li Y. Zhang C. Tian C. Mao C. Org. Biomol. Chem. 2014;12:2543. doi: 10.1039/C4OB00317A. - DOI - PubMed
    2. Shen X. Asenjo-Garcia A. Liu Q. Jiang Q. Javier Garcia de Abajo F. Liu N. Ding B. Nano Lett. 2013;13:2128. doi: 10.1021/nl400538y. - DOI - PubMed
    1. Mao C. Sun W. Shen Z. Seeman N. C. Nature. 1999;397:144. doi: 10.1038/16437. - DOI - PubMed
    2. Rajendran A. Endo M. Sugiyama H. Angew. Chem., Int. Ed. 2012;51:874. doi: 10.1002/anie.201102113. - DOI - PubMed
    3. Modi S. Nizak C. Surana S. Halder S. Krishnan Y. Nat. Nanotechnol. 2013;8:859. doi: 10.1038/nnano.2013.92. - DOI - PubMed
    1. Rich A. Nordheim A. Wang A. H. J. Annu. Rev. Biochem. 1984;53:791. doi: 10.1146/annurev.bi.53.070184.004043. - DOI - PubMed
    2. Rich A. Zhang S. Nature. 2003;4:566. - PubMed
    1. Ha S. C. Lokanath N. K. Van Quyen D. Wu C. A. Lowenhaupt K. Rich A. Kim Y. G. Kim K. K. Proc. Natl. Acad. Sci. U. S. A. 2004;101:14367. doi: 10.1073/pnas.0405586101. - DOI - PMC - PubMed
    2. Ha S. C. Lowenhaupt K. Rich A. Kim Y. G. Kim K. K. Nature. 2005;437:1183. doi: 10.1038/nature04088. - DOI - PubMed
    3. Ha S. C. Choi J. Hwang H. Y. Rich A. Kim Y. G. Kim K. K. Nucleic Acids Res. 2008;37:629. doi: 10.1093/nar/gkn976. - DOI - PMC - PubMed
    4. Bae S. Kim D. Kim K. K. Kim Y. G. Hohng S. J. J. Am. Chem. Soc. 2010;133:668. doi: 10.1021/ja107498y. - DOI - PubMed
    5. Geng J. Zhao C. Ren J. Qu X. Chem. Commun. 2010;46:7187. doi: 10.1039/C0CC02049D. - DOI - PubMed
    6. Lee Y. M. Kim H. E. Park C. J. Lee A. R. Ahn H. C. Cho S. J. Choi K. H. Choi B. S. Lee J. H. J. Am. Chem. Soc. 2012;134:5276. doi: 10.1021/ja211581b. - DOI - PubMed