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. 2019 Jan 21;9(5):2812-2815.
doi: 10.1039/c8ra10589h. eCollection 2019 Jan 18.

Ultrasensitive strips for the quadruple detection of nitrofuran metabolite residues

Wei Wu  1 , Ning Sun  2 , Simin Zhu  3 , Xiaomei Li  2 , Jitao Chen  2 , Chunzhao Liu  4 , Zhiyuan Fang  2 , Qingli Yang  1
Affiliations

Ultrasensitive strips for the quadruple detection of nitrofuran metabolite residues

Wei Wu et al. RSC Adv. .

Abstract

In this biosensor system, metabolite residues were derived by using a previous B-CBA synthesis method to label a biotin moiety for enrichment by streptavidin coated magnetic beads. Antibodies specific for derivatives were conjugated with carboxyl-modified barcode DNAs which were used as templates for strand displacement amplification (SDA). The assay can detect trace levels of 7.20 ppt of SEM, 11.58 ppt of AHD, 7.24 ppt of AOZ and 2.31 ppt of AMOZ, respectively.

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Conflict of interest statement

There are no conflicts to declare.

Figures

Scheme 1
Scheme 1. Schematic of the isothermal SDA based method for quadruple detection of four nitrofuran metabolite residues. Metabolite residue derivatives are first incubated with antibodies and SAMB, and then collected by magnetic separator. The enriched tag DNA is amplified by single direction isothermal SDA. The resulted ssDNA is further visually detected by the lateral flow biosensor.
Fig. 1
Fig. 1. The feasibility analysis of proposed method for nitrofuran metabolites detection. (A) Typical images of the biosensor for single detection of nitrofuran metabolites. (B) Typical images of the biosensor for multiplex detection of nitrofuran metabolites.
Fig. 2
Fig. 2. The sensitivity of LFB for (A) SEM, (B) AOZ, (C) AHD and (D) AMOZ detection.
Fig. 3
Fig. 3. Standard curve of the assay. Optical densities are obtained from serially diluted standard solutions from 5 ppm to 10 ppt.
See this image and copyright information in PMC

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