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. 2022 Apr 28;8(5):e09348.
doi: 10.1016/j.heliyon.2022.e09348. eCollection 2022 May.

Anti-aging potential of cookies from sea grapes in mice fed on cholesterol- and fat-enriched diet: in vitro with in vivo study

Iskari Ngadiarti  1 , Fahrul Nurkolis  2 , Matthew Nathaniel Handoko  3 , Fachruddin Perdana  4 , Happy Kurnia Permatasari  5 , Nurpudji Astuti Taslim  6 , Nelly Mayulu  7 , Defny Silvia Wewengkang  8 , Sutamara Lasurdi Noor  9 , Siti Chairiyah Batubara  10 , Melvin Junior Tanner  11 , Nindy Sabrina  12
Affiliations

Anti-aging potential of cookies from sea grapes in mice fed on cholesterol- and fat-enriched diet: in vitro with in vivo study

Iskari Ngadiarti et al. Heliyon. .

Abstract

This study determines the effect of cookies made from sea grapes (Caulerpa racemosa) on PGC-1α, total cholesterol, and blood glucose levels on mice fed with a Cholesterol- and Fat-Enriched Diet (CFED). The antioxidant activity, tyrosinase inhibition, α-glucosidase, and α-amylase inhibition is also analyzed in order to assess the in vitro anti-aging potential of sea grapes cookies. Forty male Mus muscullus albino mice weighing 20 g-30 g were used and randomly distributed into four groups of ten animals each. Group A served as a normal control (given a standard dry pellet diet), Group B was given CFED only, and mice in Groups C and D were given CFED with 100 mg and 200 mg/20 g body weight of sea grapes cookies, respectively for 4 weeks. In vitro study shows that the percentage of inhibition activity of antioxidant, L-Tyrosine, L-Dopa, α-glucosidase, and α-amylase inhibition were 45.65 ± 1.50, 8.95 ± 0.06, 21.31 ± 0.98, 77.12 ± 4.67 and 70.94 ± 0.98, respectively. This study found that group D had better activity in lowering blood glucose than group C (p < 0.0001). In addition, although there was not found significant difference between groups C and D in blood cholesterol reduction and PGC-1α (p = 0.1482), both groups experienced the same effect in total cholesterol reduction and PGC-1α in mice (significantly, p < 0001). Thus, we conclude that sea grapes cookies are proven to improve PGC-1α, total cholesterol, and blood glucose levels in mice fed with CFED. Hence, sea grapes cookies is a potential anti-aging novel-functional food.

Keywords: Antioxidant; Blood glucose; Caulerpa racemosa; Functional food; PGC-1α; Total cholesterol.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Independet-T test for Final Body Weight (g) and Weight Gain (g/day) in group A and C. (ns) = Not Significant p = 0.6962 (p > 0.05) at CI 95%.
Figure 2
Figure 2
Low Doses of Sea grapes Cookies Significantly Lower Blood Glucose. (∗∗∗∗)p < 0.0001; (∗∗)p < 0.0021; (ns)p > 0.05.
Figure 3
Figure 3
Both Doses of Sea grapes Cookies Significantly Lowered Total Blood Cholesterol. (∗∗∗∗)p < 0.0001; (∗∗)p < 0.0048; (ns)p = 0.4846.
Figure 4
Figure 4
Both Doses of Sea grapes Cookies Significantly Increase Serum PGC-1α. (∗∗∗∗)p < 0.0001; (ns)p = 0.1294.
Figure 5
Figure 5
Effects of Cookies from Sea grapes (Caulerpa racemosa) on Subject Mice.
See this image and copyright information in PMC

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