Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Oct;101(10):856-861.
doi: 10.1002/cyto.a.24564. Epub 2022 May 6.

OMIP-084: 28-color full spectrum flow cytometry panel for the comprehensive analysis of human γδ T cells

Joana Barros-Martins  1 , Elena Bruni  1   2   3 , Alina Suzann Fichtner  1 , Markus Cornberg  2   4   5 , Immo Prinz  1   2   3   5
Affiliations
Free article

OMIP-084: 28-color full spectrum flow cytometry panel for the comprehensive analysis of human γδ T cells

Joana Barros-Martins et al. Cytometry A. 2022 Oct.
Free article

Abstract

Using full spectrum flow cytometry, we designed a 28-color panel for the analysis of markers known to be associated with the γδ T cell immune response. This panel allows the classification of γδ T cell subsets via specific V gene usage (Vγ9, Vδ1, Vδ2, and Vδ3) of their T cell receptor (TCR) and according to their functional differentiation. Phenotypical surface receptors to distinguish different stages of cell maturation included CD45RA, CD27, CD28, CD127, CD57, and CD16; chemokine receptors CXCR6, CCR5, CCR6, and CX3CR1; NK-associated markers NKG2A, NKG2D, CD56, and CD161, checkpoint-inhibitor PD-1, and activating receptors CD38 and CD25. T cell lineage markers for the analysis of αβ T cells (CD4 and CD8) and MAIT cells (Vα7.2) were also included. This optimized multicolor panel allows a comprehensive immune-profiling of all main human γδ T cell subsets and is suitable for longitudinal or exploratory analysis of γδ T cell development and γδ T cell dynamics in clinical cohorts.

Keywords: Immunophenotyping; MAIT; T cells; cord blood; full spectrum flow cytometry; human PBMCs; γδ T cells.

PubMed Disclaimer

References

REFERENCES

    1. Hayday AC. γδ T cell update: adaptate orchestrators of immune surveillance. J Immunol 2019;203(2):311-20.
    1. Mikulak J, Oriolo F, Bruni E, Roberto A, Colombo FS, Villa A, et al. NKp46-expressing human gut-resident intraepithelial Vδ1 T cell subpopulation exhibits high antitumor activity against colorectal cancer. JCI Insight. 2019;4(24):1-20.
    1. Hunter S, Willcox CR, Davey MS, Chudakov DM, Oo YH, Willcox BE. Human liver infiltrating gd T cells are composed of clonally expanded circulating and tissue-resident populations. J Hepatol. 2018;69(3):654-65.
    1. Di Marco BR, Roberts NA, Dart RJ, Vantourout P, Jandke A, Nussbaumer O, et al. Epithelia use Butyrophilin-like molecules to shape organ-specific γδ T cell compartments. Cell. 2016;167(1):203-218.e17.
    1. Davey MS, Willcox CR, Hunter S, Kasatskaya SA, Remmerswaal EBM, Salim M, et al. The human Vδ2+ T-cell compartment comprises distinct innate-like Vγ9+ and adaptive Vγ9- subsets. Nat Commun. 2018;9(1):1-14.

Publication types

MeSH terms

Substances

LinkOut - more resources