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. 2022 May 6;22(1):160.
doi: 10.1186/s12903-022-02203-4.

Long non-coding RNA TFAP2A-AS1 plays an important role in oral squamous cell carcinoma: research includes bioinformatics analysis and experiments

Guo Jie  1 , ShiXiong Peng  1 , ZiFeng Cui  1 , Chen He  1 , XuPo Feng  2 , Kaicheng Yang  3
Affiliations

Long non-coding RNA TFAP2A-AS1 plays an important role in oral squamous cell carcinoma: research includes bioinformatics analysis and experiments

Guo Jie et al. BMC Oral Health. .

Abstract

Background: Oral squamous cell carcinoma (OSCC) is the most common neck and head malignancies, and the prognosis is not good. Studies shown that the long non-coding RNA (lncRNA) TFAP2A-AS1 is involved in the progression of multiple cancers. However, the role of lncRNA TFAP2A-AS1 in OSCC remains unclear. We aimed to explore the functions and expression in OSCC.

Methods: The lncRNA profiles for OSCC patients were acquired from the TCGA. Based on these data, the data mining of TFAP2A-AS1 in patients with OSCC were performed. The functions of TFAP2A-AS1 were determined by bioinformatics analysis. The expression and roles in cell growth were tested by RT-qPCR and MTS assay. Cell invasion and migration were tested by wound healing and transwell assays.

Results: The consequences displayed that TFAP2A-AS1 was upregulated in the TCGA datasets. The expression of TFAP2A-AS1 was higher in OSCC samples. Bioinformatics analysis shown that TFAP2A-AS1 might be associated with the P53 signaling pathway. Cell culture experiments indicated that deficiency of TFAP2A-AS1 inhibited cell growth, invasion, and migration, and overexpression of it could opposite results in SCC-25 cells.

Conclusion: The results suggested that TFAP2A-AS1 was overexpressed in OSCC cells, which could facilitate OSCC cell proliferation, migration, and invasion.

Keywords: Bioinformatics; Oral squamous cell carcinoma; TFAP2A-AS1; WGCNA.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Differential expression analysis of the genes in the TCGA database. A Box diagram of TFAP2A-AS1 showing differential expression between OSCC and normal groups. B Box diagram of TFAP2A showing differential expression between OSCC and normal groups. C ROC analysis of TFAP2A-AS1. D ROC analysis of TFAP2A. E Pearson correlation analysis between TFAP2A-AS1 and TFAP2A
Fig. 2
Fig. 2
WGCNA analysis of the genes in the TCGA database. A, B Repeated hierarchical clustering tree of all genes and TFAP2A-AS1is in the turquoise module. C The dendrogram and heatmap of all genes. D Interactions between these modules. E The associations between clinic traits and the modules and the correlation between turquoise module and OSCC is 0.52. F Interrelationships between TFAP2A-AS1and the genes in the turquoise module
Fig. 3
Fig. 3
Gene functional enrichment analysis of TFAP2A-AS1. A, B GO analyses by GSEA. C, D KEGG analyses of by GSEA
Fig. 4
Fig. 4
The expression of TFAP2A-AS1. A Relative expression levels of TFAP2A-AS1 in the Clinical OSCC samples which included 64 OSCC tissues and 64 paired tissues from the adjacent normal tissues. B The relationship between the expression of TFAP2A-AS1 and clinicopathologic features of OSCC tissues C The expression of TFAP2A-AS1 in OSCC cell lines. D Validation of TFAP2A-AS1 knock down in OSCC cell lines. E Validation of TFAP2A-AS1 over-expression in OSCC cell lines. F Validation of TFAP2A-AS1 knock down in OSCC cell lines 15 days after transfection. G Validation of TFAP2A-AS1 over-expression in OSCC cell lines 15 days after transfection. *P < 0.05 and **P < 0.01
Fig. 5
Fig. 5
MTS and CCK-8 analysis are used to study the effect of knocking down or overexpression of TFAP2A-AS1 on cell proliferation in the cell line. A The results of MTS assay in TFAP2A-AS1 knocked down cell line. B The results of MTS assay in TFAP2A-AS1 over-expression cell line. C The results of clone formation experiment of TFAP2A-AS1 knocked down cell line. D The results of clone formation experiment of TFAP2A-AS1 over-expression cell line
Fig. 6
Fig. 6
Wound healing assay of TFAP2A-AS1. A Wound healing assay of TFAP2A-AS1 knock down in OSCC cell lines. B Wound healing assay of TFAP2A-AS1 over-expression in OSCC cell lines. The Y-axis represents cell migration distance
Fig. 7
Fig. 7
Transwell assay of TFAP2A-AS1. A Transwell assay of TFAP2A-AS1 knock down in OSCC cell lines. B Transwell assay of TFAP2A-AS1 over-expression in OSCC cell lines
Fig. 8
Fig. 8
Effect of TFAP2A-AS1 knockdownon or overexpression on the growth (A) and weight (B) of xenografts derived from OSCC cells. **P < 0.001
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