Development of a multifunctional injectable temperature-sensitive gelatin-based adhesive double-network hydrogel

Abstract

Gelatin-based bioadhesives are suitable for the treatment of wounds due to their inherent biocompatibility, lack of immunogenicity, and potential for modification. However, common limitations with such adhesives include their adhesive strength and versatility. In the present study, a multifunctional injectable temperature-sensitive gelatin-based adhesive double-network hydrogel (DNGel) was engineered using facile dual-syringe methodology. An integrative crosslinking strategy utilized the complexation of catechol-Fe³⁺ and NIPAAm-methacryloyl. As anticipated, the DNGel exhibited multifunctional therapeutic properties, namely temperature-sensitivity, mechanical flexibility, good adhesive strength, injectability, self-healing capability, antibacterial activity, and the capability to enable hemostasis and wound healing. The bioinspired dynamic double-network was stabilized by a number of molecular interactions between components in the DNGel, providing multifunctional therapeutic performance. In addition, comprehensive in vitro and in vivo testing confirmed that the adhesive hydrogel exhibited effective antihemorrhagic properties and accelerated wound healing by the promotion of revascularization, representing considerable potential as a next-generation multifunctional smart adhesive patch.

Keywords: Bioadhesives; Facile dual-syringe methodology; Gelatin; Multifunctional therapeutic properties.