Comparative Study
doi: 10.1083/jcb.77.3.714.
Localization of actin in Dictyostelium amebas by immunofluorescence
- PMID: 355262
- PMCID: PMC2110147
- DOI: 10.1083/jcb.77.3.714
Item in Clipboard
Comparative Study
Localization of actin in Dictyostelium amebas by immunofluorescence
J Cell Biol.
1978 Jun.
Abstract
Antibody prepared against avian smooth muscle actin has been used to localize actin in the slime mold, Dictyostelium discoideum. The distribution of actin in migrating cells is different from that in feeding cells. Migrating amebas display fluorescence primarily in advancing regions whereas feeding amebas show uniform fluorescence throughout. The reaction is specific for actin since the fluorescence observed is blocked when the antibody is absorbed by actin purified from avian skeletal muscle, human platelets, and Dictyostelium. These results, in addition to describing the distribution of actin in D. discoideum, demonstrate that actins from these diverse sources share at least one common antigenic determinant.
Similar articles
-
The Dictyostelium discoideum 30,000-dalton protein is an actin filament-bundling protein that is selectively present in filopodia.J Cell Biol. 1987 Jun;104(6):1539-51. doi: 10.1083/jcb.104.6.1539. J Cell Biol. 1987. PMID: 3294856 Free PMC article.
-
Structural and biochemical aspects of cell motility in amebas of Dictyostelium discoideum.J Cell Biol. 1977 Feb;72(2):339-50. doi: 10.1083/jcb.72.2.339. J Cell Biol. 1977. PMID: 188829 Free PMC article.
-
Supramolecular forms of actin from amoebae of Dictyostelium discoideum.J Biol Chem. 1975 Sep 25;250(18):7485-91. J Biol Chem. 1975. PMID: 1172503
-
Slime mold lectins.Int Rev Cytol. 1982;75:61-99. doi: 10.1016/s0074-7696(08)61002-5. Int Rev Cytol. 1982. PMID: 7049989 Review. No abstract available.
-
Synthesis and translation of messenger RNA during differentiation of the cellular slime mold Dictyostelium discoideum.Symp Soc Dev Biol. 1976;(34):75-103. Symp Soc Dev Biol. 1976. PMID: 798345 Review. No abstract available.
Cited by
-
Association of the cyclic AMP chemotaxis receptor with the detergent-insoluble cytoskeleton of Dictyostelium discoideum.J Cell Biol. 1984 Feb;98(2):584-95. doi: 10.1083/jcb.98.2.584. J Cell Biol. 1984. PMID: 6693497 Free PMC article.
-
Ligand-induced changes in the location of actin, myosin, 95K (alpha-actinin), and 120K protein in amebae of Dictyostelium discoideum.J Cell Biol. 1985 Jun;100(6):1884-93. doi: 10.1083/jcb.100.6.1884. J Cell Biol. 1985. PMID: 3889011 Free PMC article.
-
Abundance, relative gelation activity, and distribution of the 95,000-dalton actin-binding protein from Dictyostelium discoideum.J Cell Biol. 1983 Jul;97(1):178-85. doi: 10.1083/jcb.97.1.178. J Cell Biol. 1983. PMID: 6863390 Free PMC article.
-
Filopodelike projections induced with dimethyl sulfoxide and their relevance to cellular polarity in Dictyostelium.J Cell Biol. 1983 Mar;96(3):857-65. doi: 10.1083/jcb.96.3.857. J Cell Biol. 1983. PMID: 6682113 Free PMC article.
-
Location of actin, myosin, and microtubular structures during directed locomotion of Dictyostelium amebae.J Cell Biol. 1984 Feb;98(2):382-90. doi: 10.1083/jcb.98.2.382. J Cell Biol. 1984. PMID: 6319425 Free PMC article.
