Is phylloquinone an obligate electron carrier in photosystem I?

Abstract

Comparative quantitative analysis of phylloquinone content and photochemically competent P-700 has been performed on photosystem I particles subjected to photolysis with ultraviolet irradiation. Nonirradiated control particles exhibit a phylloquinone/P-700 stoichiometry of 1.9 +/- 0.2. Photolysis of the photosystem I particles induces a progressive depletion of phylloquinone, however, photochemistry as assayed at room temperature by the photooxidation of P-700 is unaffected. These data are not consistent with the assignment of phylloquinone as a functional intermediate at room temperature between P-700 and the iron-sulfur clusters, center A and center B.