[Talin1 is highly expressed in the fallopian tube and chorionic villi to promote trophoblast invasion in tubal pregnancy]

Abstract

Objective: To investigate the expression of Talin1 in the fallopian tube and chorionic villi in patients with tubal pregnancy and its role in regulating invasion and migration of trophoblasts.

Methods: Immunohistochemistry and Western blotting were used to detect the localization and expression level of Talin1 in the fallopian tube and chorionic villi in patients with tubal pregnancy and in women with normal pregnancy. In the cell experiment, HTR-8/SVneo cells was transfected with Talin1 siRNA and the changes in cell invasion and migration were assessed using scratch assay and Transwell assay. The expressions of MMP-2, MMP-9, N-cadherin and Snail in the transfected cells were detected by qRT-PCR and Western blotting.

Results: Positive expression of Talin1 was detected in both normal fallopian tube tissues and tissues from women tubal pregnancy, and its expression was localized mainly in the cytoplasm of cilia cells. The expression level of Talin1 was significantly higher in both the fallopian tube and chorionic villi in women with tubal pregnancy than in normal fallopian tube and chorionic villi samples (P < 0.01). In HTR-8/SVneo cells, transfection with Talin1 siRNA significantly inhibited cell invasion (P < 0.01) and migration (P < 0.05), down-regulated the expression of N-cadherin, MMP-2 and Snail (P < 0.05), and up-regulated the expression of MMP-9 in the cells (P < 0.05).

Conclusion: The expression of Talin1 in the fallopian tube and chorionic villi is significantly increased in women with tubal pregnancy, suggesting the association of Talin1-regulated trophoblast cell invasion with the occurrence of tubal pregnancy.

Keywords: Talin1; invasion; tubal pregnancy.

图 1

人输卵管组织HE染色 HE staining of human fallopian tube tissue.A: Fallopian tube tissue with tubal pregnancy (Original magnification: × 40). B: Fallopian tube tissue with normal pregnancy(×100). C: Normal tubal tissue (×40). D: Normal tubal tissue(×100).

图 2

Talin1在输卵管粘膜上皮的表达定位 Expression and localization of Talin1 in the epithelium of the fallopian tube. A: Fallopian tube tissue with tubal pregnancy (× 40). B: Fallopian tube tissue with normal pregnancy (×200). C: Normal tubal tissue (×40). D: Normal tubal tissue (×200).

图 3

Talin1在输卵管粘膜上皮的蛋白表达 Protein expression of Talin1 in the epithelium of fallopian tube in different groups. A: Talin1 expression detected by Western blotting. B: Histogram of Talin1 protein expression. NT: Normal fallopian tube group. GT: Gestational fallopian tube group. ^**P < 0.01 vs NT group.

图 4

Talin1在绒毛组织上的蛋白表达 Protein expression of Talin1 in the villi tissue in different groups. A: Talin1 expression identified by Western blotting. B: Histogram of Talin1 protein expression. NV: Normal intrauterine pregnancy villi group. TV: Tubal pregnancy villi group. ^**P < 0.01 vs NV group.

图 5

siRNA对Talin1的表达抑制效果 Inhibitory effect of siRNA on Talin1 expression. A: mRNA expression identified by qRT-PCR; B: Protein expression identified by Western blotting. ^**P < 0.01 vs control group.

图 6

各组细胞中N-cadherin、MMP-2、Snail、MMP-9的mRNA和蛋白表达水平 mRNA and protein expression levels of N-cadherin, MMP-2, Snail and MMP-9 in each group. A: mRNA expression identified by qRT-PCR. B: Protein expression identified by Western blotting. ^*P < 0.05 vs control group.

图 7

Si-Talin1对HTR-8/SVneo细胞迁移的影响 Effect of Si-Talin 1 on HTR-8/SVneo cell migration. A: Relative migration distance. B: Wound healing assay. ^*P < 0.05 vs control group (scale bar: 200 μm).

图 8

Si-Talin1对HTR-8/SVneo细胞侵袭的影响 Effect of Si- Talin 1 on HTR- 8/SVneo cell invasion. A: Transwell assay. B: Migrated cells. ^**P < 0.01 vs control group (scale bar: 200 μm).