Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Aug;75(2):363-367.
doi: 10.1111/lam.13735. Epub 2022 May 18.

Phenotypic and genotypic approach to characterize a Trueperella pecoris strain isolated from necrotic vestibulitis of a camel (Camelus dromedarius)

M F E Ahmed  1   2 , M Alssahen  3 , C Lämmler  3 , C Hoffmann  3 , E Prenger-Berninghoff  3 , C Ewers  3 , M Plötz  4 , A Abdulmawjood  4
Affiliations

Phenotypic and genotypic approach to characterize a Trueperella pecoris strain isolated from necrotic vestibulitis of a camel (Camelus dromedarius)

M F E Ahmed et al. Lett Appl Microbiol. 2022 Aug.

Abstract

The present study was designed to characterize phenotypically and genotypically a Trueperella (T.) pecoris strain isolated from necrotic vestibulitis of a 10-year-old camel (Camelus dromedarius). The species identity of T. pecoris 203/7 investigated in the present study could be confirmed by phenotypic properties and by phylogenetic analyses based on partial sequencing of the 16S ribosomal RNA (rRNA) gene, the 16S-23S rDNA intergenic spacer region, the glyceraldehyde 3-phosphate dehydrogenase encoding gene gap, elongation factor Tu encoding gene tuf and the target gene rpoB encoding the β-subunit of bacterial RNA polymerase. T. pecoris strain 203/7 was grouped within the genus Trueperella in the family Arcanobacteriaceae. The 16S rRNA gene analysis showed a sequence identity of 99·9% to reference strain T. pecoris DSM 111392T . The present isolate was clearly identified as T. pecoris, the most recently described species of the genus Trueperella. Strain T. pecoris 203/7 was isolated in moderate numbers from necrotic vestibulitis of the camel and could be of some importance for the infectious process. However, the investigated strain represents the first isolation of T. pecoris from a camel.

Keywords: 16S rRNA gene; 16S-23S rDNA intergenic spacer region; T. pecoris; camel; gap; rpoB; tuf.

PubMed Disclaimer

References

    1. Ahmed, M., Alssahen, M., Lämmler, C., Eisenberg, T., Plötz, M. and Abdulmawjood, A. (2020) Studies on Trueperella pyogenes isolated from an okapi (Okapia johnstoni) and a royal python (Python regius). BMC Vet Res 16, 1-8.
    1. Alssahen, M., Peters, M., Rau, J., Hassan, A.A., Sammra, O., Lämmler, C., Prenger-Beringhoff, E., Plötz, M. et al. (2020) Phenotypic and genotypic approach to characterize a Trueperella pyogenes strain isolated from an Eurasian lynx (Lynx lynx). Berl Münch Tierärztl Wochenschr 133, 1-5.
    1. Bastin, B., Bird, P., Benzinger, M.J., Jr., Crowley, E., Agin, J., Goins, D., Sohier, D., Timke, M. et al. (2019) Confirmation and identification of Salmonella spp., Cronobacter spp., and other Gram-negative organisms by the Bruker MALDI Biotyper method: collaborative study method extension to include Campylobacter species, revised first action 2017.09. J AOAC Int 102, 1595-1616.
    1. Eisenberg, T., Nagib, S., Hijazin, M., Alber, J., Lämmler, C., Hassan, A.A., Timke, M., Kostrzewa, M. et al. (2012) Trueperella pyogenes as cause of a facial abscess in a grey slender Loris (Loris lydekkerianus nordicus) - a case report. Berl Münch Tierärztl Wochenschr 125, 407-410.
    1. Hassan, A.A., Ülbegi-Mohyla, H., Kanbar, T., Alber, J., Lämmler, C., Abdulmawjood, A., Zschöck, M. and Weiss, R. (2009) Phenotypic and genotypic characterization of Arcanobacterium haemolyticum isolates from infections of horses. J Clin Microbiol 47, 124-128.

LinkOut - more resources