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. 2022 Apr;29(2):69-79.
doi: 10.21315/mjms2022.29.2.7. Epub 2022 Apr 21.

Impact of Rapid Eye Movement Sleep Deprivation on Pain Behaviour and Oxidative Stress in the Thalamus: Role of Tualang Honey Supplementation

Mohd Shafie Anis Syahirah  1 , Abd Aziz Che Badariah  1 , Long Idris  2 , Siran Rosfaiizah  3 , Noordin Liza  1
Affiliations

Impact of Rapid Eye Movement Sleep Deprivation on Pain Behaviour and Oxidative Stress in the Thalamus: Role of Tualang Honey Supplementation

Mohd Shafie Anis Syahirah et al. Malays J Med Sci. 2022 Apr.

Abstract

Background: Insufficient sleep alters the body's physiological functions. This study investigated whether oxidative stress (OS) in the thalamus was correlated with the pain behaviour score in the rapid eye movement (REM) sleep-deprived rat model.

Methods: Four groups of Sprague-Dawley rats were included in the study (n = 6): i) control; ii) REM sleep-deprived rats for 72 h (REMsd); iii) REM sleep-deprived rats for 72 h pretreated with Tualang honey (REMsd-H) and iv) tank control (TC). Following the intervention, 1% formalin was injected on the right hind paw and pain behaviour was recorded for 1 h. OS markers of glutathione (GSH), glutathione reductase (GR), superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) in the thalamus were measured.

Results: REM sleep deprivation increased pain behaviour scores in phase II of the formalin test with decreased GSH, GR, SOD and CAT. The MDA level was significantly higher in the REMsd compared to REMsd-H. There was an inverse correlation between pain behaviour scores and GSH, GR and SOD levels. A positive correlation was found between the pain behaviour score and the MDA level.

Conclusion: OS levels were significantly correlated with the pain behaviour score in the REMsd rats. Tualang honey administration reduced pain behaviour score and OS in the thalamus.

Keywords: REM sleep deprivation; Tualang honey; oxidative stress biomarkers; pain behaviour score.

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Conflict of interest statement

Conflict of Interest None.

Figures

Figure 1
Figure 1
Effects of REM sleep deprivation and Tualang honey administration on pain behaviour score in each phase of formalin test (n = 6). REM sleep deprivation has significantly increased pain behaviour score in late phase 2 (bP < 0.001) compared to Control. There were significant differences when compared between REMsd-H and REMsd groups during early and late phase 2 (bP < 0.001). The difference was also significant when compared between REMsd-H and TC groups (aP < 0.01).
Figure 2
Figure 2
Scatter plots of correlations between OS parameters: A) glutathione, B) glutathione reductase, C) superoxide dismutase, D) catalase and E) malondialdehyde and pain behaviour score
See this image and copyright information in PMC

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