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. 2022 Apr;29(2):55-68.
doi: 10.21315/mjms2022.29.2.6. Epub 2022 Apr 21.

The Characterisation of Diarrhoeagenic Verotoxin Producing Non-O157 Escherichia coli among Young Children in Kuantan, Malaysia

Md Fazlul Karim Khan  1 , Shah Samiur Rashid  1 , Aizi Nor Mazila Ramli  1 , Muhammad Nomani Kabir  2 , Mohammad Nazmul Hasan Maziz  3
Affiliations

The Characterisation of Diarrhoeagenic Verotoxin Producing Non-O157 Escherichia coli among Young Children in Kuantan, Malaysia

Md Fazlul Karim Khan et al. Malays J Med Sci. 2022 Apr.

Abstract

Background: Diarrhoeagenic verotoxin producing non-O157 Escherichia coli (VTEC) are associated with endemic infantile diarrhoea-causing morbidity and mortality worldwide. VTEC can also cause severe illness and has an impact on outbreaks, especially in developing countries. This study aims to investigate the prevalence and characterisation of VTEC and their association in causing infectious diarrhoea among Malaysian children.

Methods: Standard microbiological techniques identified a total of 137 non-repeated, clinically significant E. coli isolates. Serological assays discerned non-O157 E. coli serogroup, subjected to virulence screen (VT1 and VT2) by a polymerase chain reaction (PCR).

Results: Different PCR sets characterised the 49 clinical isolates of sorbitol positive non-O157 E. coli. Twenty-nine isolates harboured verotoxin genes associated with diarrhoea among children (≤ 5 years old). Among the 29 (59.18%) strains of verotoxin producing E. coli, genotypes VT1 and VT2 were detected in 21 (42.85%) and 5 (10.20%) isolates respectively, while both VT1 and VT2 genes were confirmed in 3 (6.12%) isolates.

Conclusion: This study evaluates on the prevalence, serological characteristics and antimicrobial susceptibility patterns of VTEC diarrhoea affected children (≤ 5 years old). Besides, the prevalence of verotoxin gene was determined as a root cause of diarrhoea among Malaysian children.

Keywords: Escherichia coli; Malaysia; children; diarrhoea; non-O157 E. coli.

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Conflict of interest statement

Conflict of Interest None.

Figures

Figure 1
Figure 1
Formation of non-O157 E. coli on CT-SMAC culture plate media
Figure 2
Figure 2
Confirmation of VTEC
Figure 3
Figure 3
Antibiogram patterns of VTEC
See this image and copyright information in PMC

References

    1. Rajendran P, Ajjampur SS, Chidambaram D, Chandrabose G, Thangaraj B, Sarkar R, et al. Pathotypes of diarrheagenic Escherichia coli in children attending a tertiary care hospital in South India. Diagn Microbiol Infect Dis. 2010;68(2):117–122. doi: 10.1016/j.diagmicrobio.2010.06.003. - DOI - PMC - PubMed
    1. Walker CL, Aryee MJ, Boschi-Pinto C, Black RE. Estimating diarrhea mortality among young children in low and middle income countries. PloS ONE. 2012;7(1):e29151. doi: 10.1371/journal.pone.0029151. - DOI - PMC - PubMed
    1. Gurpreet K, Tee GH, Amal NM, Paramesarvathy R, Karuthan C. Incidence and determinants of acute diarrhoea in Malaysia: a population-based study. J Health Popul. Nutr. 2011;29(2):103–112. doi: 10.3329/jhpn.v29i2.7814. - DOI - PMC - PubMed
    1. Colello R, Caceres ME, Ruiz MJ, Sanz M, Etcheverria AI, Padola NL. From farm to table: follow-up of Shiga toxin-producing Escherichia coli throughout the pork production chain in Argentina. Front Microbiol. 2016;7:93. doi: 10.3389/fmicb.2016.00093. - DOI - PMC - PubMed
    1. Public Health England. Epidemiology of non-O157 Shiga toxin producing Escherichia coli (STEC) 2018. Available at: https://assets.publishing.service.gov.uk/government/uploads/system/uploa....

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