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. 2022 Apr;29(2):31-42.
doi: 10.21315/mjms2022.29.2.4. Epub 2022 Apr 21.

Association of Variants in COMT, RASSF1 and GPM6A with the Risk of Paranoid Schizophrenia Patients in Prof HB Saanin Psychiatric Hospital, West Sumatra, Indonesia

Luluk Yunaini  1 , Edwina Khairat  2
Affiliations

Association of Variants in COMT, RASSF1 and GPM6A with the Risk of Paranoid Schizophrenia Patients in Prof HB Saanin Psychiatric Hospital, West Sumatra, Indonesia

Luluk Yunaini et al. Malays J Med Sci. 2022 Apr.

Abstract

Background: Schizophrenia is a multifactorial disease in which genetic factors play a greater role than other factors. The genes of importance in schizophrenia patients are the genes that encode for neurotransmitters associated with low minor allele frequency (MAF) scores. This study was aimed to determine the association of genetic variations in catechol-O-methyl transferase (COMT), Ras association domain family member 1 (RASSF1) and glycoprotein M6A (GPM6A) with the risk of paranoid schizophrenia (PS) in patients admitted to Prof HB Saanin Psychiatric Hospital, West Sumatra, Indonesia.

Methods: Genotyping analysis through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and PCR-amplification refractory mutation system (ARMS) was performed in 100 PS patients and 100 healthy controls. Chi-square and Fisher's exact tests were used to compare the frequencies of genotype and allotype between the PS and control groups. Odds ratio (OR) with 95% confidence interval (95% CI) were calculated to determine the relative risk of PS with respect to genetic variations.

Results: Polymorphism rs13142920 in GPM6A was associated with significantly elevated risk of PS (P = 0.020; OR = 1.60 [95% CI: 1.08, 2.39]). However, COMT rs4680 and RASSF1 rs2073499 polymorphisms were not significantly associated with PS.

Conclusion: The GPM6A rs13142920 polymorphism holds great potential as a genetic marker in PS patients.

Keywords: COMT; GPM6A; RASSF1; paranoid schizophrenia; polymorphism.

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Conflict of interest statement

Conflict of Interest None.

Figures

Figure 1
Figure 1
Agarose gel electrophoresis of PCR-RFLP products for the RASSF1 rs2073499. Lane M represents 50 bp DNA ladder marker; lanes 1, 2, 5, 6, 7 and 9 represent GA genotype; lane 3 represents AA genotype; lane 8 represents GG genotype; and lane 4 represents negative control
Figure 2
Figure 2
Tetra-primer PCR-ARMS method (26) (with modification). Different colours represent different primers involved in the PCR reaction. Blue: outer primer, orange: inner specific primer for C allele, and green: inner specific primer for A allele
Figure 3
Figure 3
Agarose gel electrophoresis of ARMS-PCR products for the GPM6A rs13142920. Lane M represents 100 bp DNA ladder marker; lanes 1, 2 and 4–6 represent CC genotype; lane 3 represents CA genotype; and lane 7 represents AA genotype
Figure 4
Figure 4
Agarose gel electrophoresis of ARMS-PCR products for the COMT rs4680. Lane M represents 100 bp DNA ladder marker; lane 1 represents GA genotype; lane 2 represents AA genotype; and lane 3 represents GG genotype
See this image and copyright information in PMC

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