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. 2019 Aug 30;9(47):27398-27405.
doi: 10.1039/c9ra04410h. eCollection 2019 Aug 29.

The first demonstration of a novel isolated fungus Eutypella sp. BJ associated with the biodegradation of polyvinyl alcohol

Yiling Deng  1 , Chenyuan Wang  1 , Yalan Liu  1 , Ping Chen  1 , Xiaoshan Lin  1 , Yi Zhang  1
Affiliations

The first demonstration of a novel isolated fungus Eutypella sp. BJ associated with the biodegradation of polyvinyl alcohol

Yiling Deng et al. RSC Adv. .

Abstract

The aim of this work is to study the potential degradation of polyvinyl alcohol (PVA) by a novel fungus Eutypella sp. BJ isolated from soil compost. When it was cultured on a semi-synthetic medium containing PVA at 30 °C and 160 rpm for 8 days, the removal rates of PVA 1788, 1799 and 2488 reached 87.40%, 86.31% and 44.80%, respectively. Gel permeation chromatography (GPC) analysis revealed significant reductions of the number average molecular weight and the weight average molecular weight of PVA 1788 from 47 358 to 13 345 and from 71 387 to 24 238, respectively, after 8 days. Fourier transform infrared spectroscopy (FTIR) indicated that some substances containing carbonyl groups (likely aldehydes or ketones) might have been produced during the biodegradation process. These results indicate that the isolate has potential for degrading PVA. This study provides the first demonstration that Eutypella has the ability to assimilate PVA.

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Conflict of interest statement

All contributing authors declare that they have no conflicts of interest.

Figures

Fig. 1
Fig. 1. Agar plate assay for PVA degradation by Eutypella sp. BJ.
Fig. 2
Fig. 2. Phylogenetic analysis of the Eutypella sp. BJ based on ITS sequence.
Fig. 3
Fig. 3. Photographs of 7 days fungal cultures incubated in Petri plates. (a) Growth of Eutypella sp. BJ on CYA medium; (b) growth of Eutypella sp. BJ on MEA medium; (c) growth of Eutypella sp. BJ on PDA medium.
Fig. 4
Fig. 4. Micrographs of Eutypella sp. BJ (×1000).
Fig. 5
Fig. 5. Biodegradation curves of PVA samples with different degrees of polymerization and saponification recorded in biodegradation tests. (a) biodegradation curve of PVA 1788; (b) biodegradation curve of PVA 2488; (c) biodegradation curve of PVA 1799.
Fig. 6
Fig. 6. Molecular weight distribution of PVA 1788 in the semi-synthetic medium as recorded at different incubation times.
Fig. 7
Fig. 7. FTIR spectra of PVA 1788 degradation at different incubation times.
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