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. 2019 Sep 30;9(53):30760-30767.
doi: 10.1039/c9ra05868k. eCollection 2019 Sep 26.

Effects of nanobubble water on the growth of Lactobacillus acidophilus 1028 and its lactic acid production

Zitao Guo  1 , Xuezhi Wang  1 , Hanxiao Wang  1 , Bo Hu  2 , Zhongfang Lei  1 , Motoyoshi Kobayashi  1 , Yasuhisa Adachi  1 , Kazuya Shimizu  1 , Zhenya Zhang  1
Affiliations

Effects of nanobubble water on the growth of Lactobacillus acidophilus 1028 and its lactic acid production

Zitao Guo et al. RSC Adv. .

Abstract

Nanobubble water (NBW) has been applied in various fields due to the unique properties of nanobubbles (NBs) including long-term stability, negative zeta potential and generation of free radicals. In this study, the performance of four kinds of NBW from different gases (air, N2, H2, and CO2) in addition to deionized water (DW) were investigated and compared in terms of the growth of the probiotic Lactobacillus acidophilus 1028. The NB density, size distribution, zeta potential, pH and dissolved oxygen (DO) of the NBW were firstly investigated. Results indicate that N2-NBW had the highest absolute value of zeta potential and NB density (-25.3 ± 5.43 mV and 5.73 ± 1.0 × 107 particles per mL, respectively), while the lowest was detected in CO2-NBW (-6.96 ± 2.36 mV and 3.39 ± 1.73 × 107 particles per mL, respectively). With the exception of CO2-NBW, all the other types of NBW showed promotion effects on the growth of the strain at the lag and logarithmic phases. Among them, N2-NBW demonstrated the best performance, achieving the highest increase ratio of 51.1% after 6 h cultivation. The kinetic models (Logistic and Gompertz) indicate that the culture with N2-NBW had the shortest lag phase and the maximum specific growth rate when compared to the H2-NBW and DW groups under the same cultivation conditions. Preliminary analysis on the mechanisms suggested that these effects were related to the properties (zeta potential and density) of the NBs, which might affect the transport of substances. This study suggests that NBW has the potential for promoting the production efficiency of probiotics via fermentation.

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Conflict of interest statement

There are no conflicts to declare

Figures

Fig. 1
Fig. 1. Growth of LA1028 under the addition of different gas NBW at different volume percentages. (A) Air-NBW, (B) CO2-NBW, (C) H2-NBW, and (D) N2-NBW. Y in NBW-Y denotes the volume percentage of NBW in the test was Y%.
Fig. 2
Fig. 2. Comparison between N2-NBW and H2-NBW effects on the growth of LA1028 under the same condition (90% volume percentage). (A) The growth curve, (B) lactic acid concentration, (C) glucose concentration. IR, increase ratio; H/D, H2-NBW/DW test; N/D, N2-NBW/DW test.
Fig. 3
Fig. 3. The possible mechanisms for the promotion effects of NBW on the growth of LA 1028.
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