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. 2022 May 9;204(6):306.
doi: 10.1007/s00203-022-02915-9.

Mixed bacterial consortium can hamper the efficient degradation of crude oil hydrocarbons

Obianuju Obiajulu Nnabuife  1 , James Chukwuma Ogbonna  2 , Chukwudi Anyanwu  2 , Anthony Chibuogwu Ike  2 , Chibuzor Nwadibe Eze  2 , Simeon Chukwuemeka Enemuor  2
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Mixed bacterial consortium can hamper the efficient degradation of crude oil hydrocarbons

Obianuju Obiajulu Nnabuife et al. Arch Microbiol. .

Abstract

Crude oil degradation efficiency can be improved because of co-metabolism that exists when bacterial consortium is applied. However, because of possible vulnerability to environmental conditions and/or antagonistic interactions among members of the consortium, the degradation efficiency can be hampered. In this laboratory-based study, the biodegradation potentials of pure bacterial isolates namely Pseudomonas aeruginosa strain W15 (MW320658), Providencia vermicola strain W8 (MW320661) and Serratia marcescens strain W13 (MW320662) earlier isolated from crude oil-contaminated site and their consortium were evaluated using 3% crude oil-supplemented Bushnell Haas media. The efficiency was evaluated based on the viable cell count, biosurfactant analyses, percentage hydrocarbon degradation using gravimetric analysis and gas chromatography-mass spectrophotometry (GC-MS) analysis. There was decline in the population of W13 and predominance of W15 in the consortium as the incubation period progressed. Accelerated biodegradation of the crude oil hydrocarbons through co-metabolism was not achieved with the consortium; neither was there any improved resilience nor resistance to environmental changes of strain W13. The GC-MS analyses showed that the highest degradation was produced by W15 (48.23%) compared to W8 (46.04%), W13 (45.24%) and the Consortium (28.51%). The biodegradation of the crude oil hydrocarbons by W15, W8, W13 axenic cultures and their consortium treatments demonstrated that the bacterial constituent in a consortium can influence the synergistic effect that improves bioremediation. Future research that focuses on evaluating possible improvement in bioremediation through maintenance of diversity by continuous bioaugmentation using vulnerable but efficient degraders in a consortium is necessary to further understand the application of consortia for bioremediation improvement.

Keywords: Axenic culture; Consortium; Crude oil; Degradation; Hydrocarbons.

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