Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2022 Apr 30:2022:7378379.
doi: 10.1155/2022/7378379. eCollection 2022.

Cryopreservation Techniques for Ram Sperm

Amit Saha  1 , Mohammad Asaduzzaman  1   2 , Farida Yeasmin Bari  1
Affiliations
Review

Cryopreservation Techniques for Ram Sperm

Amit Saha et al. Vet Med Int. .

Abstract

Germplasm storage and transportation in artificial insemination (AI) and other advanced technologies are facilitated by cryopreservation. In reproduction, the cryopreservation of sperm allows it to be transported across vast distances and used even after the sire's death. However, the technique of cryopreservation might damage sperm and limit their activity. Several cryobiological investigations have reported that the integrity of the sperm membrane is frequently involved in the physical and biological elements that affect sperm survival at low temperatures during the cryopreservation process. However, successful cryopreservation of ram sperm is still a work in progress because a considerable percentage of sperm do not survive the freezing and thawing process. Sperms are destroyed during cryopreservation of semen due to varying concentrations of cryoprotective chemicals and if semen is not cooled at optimal cooling rates. Hence, it is crucial to know the optimum cooling rates with freezing and thawing protocols for maximum recovery of viable and functional sperm cells for a successful cryo-freezing of ram spermatozoa. Therefore, the current study compiled and compared the research on the impact of different cryopreservation procedures, cooling rates, equilibration time, and thawing protocols on post-thaw ram semen quality.

PubMed Disclaimer

Conflict of interest statement

The manuscript is concerned with all authors and the authors declare no conflicts of interest.

References

    1. Benson J. D., Woods E. J., Walters E. M., Critser J. K. The cryobiology of spermatozoa. Theriogenology . 2012;78(8):1682–1699. doi: 10.1016/j.theriogenology.2012.06.007. - DOI - PubMed
    1. Salamon S., Maxwell W. M. C. Frozen storage of ram semen I. processing, freezing, thawing and fertility after cervical insemination. Animal Reproduction Science . 1995;37(3-4):185–249. doi: 10.1016/0378-4320(94)01327-i. - DOI
    1. Pontbriand D., Howard J. G., Schiewe M. C., Stuart L. D., Wildt D. E. Effect of cryoprotective diluent and method of freeze-thawing on survival and acrosomal integrity of ram spermatozoa. Cryobiology . 1989;26(4):341–354. doi: 10.1016/0011-2240(89)90058-8. - DOI - PubMed
    1. Tekin N., Uysal O., Akcay E., Yavas I. Effects of different taurine doses and freezing rate on freezing of row semen. Ankara Üniversitesi Veteriner Fakültesi Dergisi . 2006;53
    1. Chelucci S., Pasciu V., Succu S., et al. Soybean lecithin–based extender preserves spermatozoa membrane integrity and fertilizing potential during goat semen cryopreservation. Theriogenology . 2015;83(6):1064–1074. doi: 10.1016/j.theriogenology.2014.12.012. - DOI - PubMed

LinkOut - more resources