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. 2018 Jun 28;8(42):23578-23584.
doi: 10.1039/c8ra02176g. eCollection 2018 Jun 27.

On the synergy between silver nanoparticles and doxycycline towards the inhibition of Staphylococcus aureus growth

Heloiza F O Silva  1 , Rayane P de Lima  1 , Fernanda S L da Costa  1 , Edgar P Moraes  1 , Maria C N Melo  2 , Celso Sant'Anna  3 , Mateus Eugênio  3 , Luiz H S Gasparotto  1
Affiliations

On the synergy between silver nanoparticles and doxycycline towards the inhibition of Staphylococcus aureus growth

Heloiza F O Silva et al. RSC Adv. .

Abstract

In a previous paper (RSC Adv., 2015, 5, 66886-66893), we showed that the combination of silver nanoparticles (NanoAg) with doxycycline (DO) culminated in an increased bactericidal activity towards E. coli. Herein we further investigated the metabolic changes that occurred on Staphylococcus aureus upon exposure to NanoAg with the help of attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) coupled with multivariate data analysis. It has been discovered that the combination of DO with NanoAg produced metabolic changes in S. aureus that were not simply the overlap of the treatments with DO and NanoAg separately. Our results suggest that DO and NanoAg act synergistically to impede protein synthesis by the bacteria.

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Conflict of interest statement

There are no conflicts to declare.

Figures

Fig. 1
Fig. 1. (A) UV-VIS spectra of DO mixed with AgNPs (red curve) and the mathematical combination of the DO and NanoAg pure spectra. (B) Chemical structure of doxycycline.
Fig. 2
Fig. 2. TEM images of (A) NanoAg and (B) NanoAg mixed with doxycycline.
Fig. 3
Fig. 3. Average spectrum for each original class control, DO, DO + NanoAg and NanoAg.
Fig. 4
Fig. 4. Multivariate data analysis of selected variables in the samples. (A) Principal Component Analysis (PCA) of variables by the four classes and (B–D) PLS-DA by pairs.
Fig. 5
Fig. 5. Mean average absorbances of the control, DO, NanoAg and DO + NanoAg classes presented in the six regions used in the discrimination by PLS-DA.
See this image and copyright information in PMC

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References

    1. Alanis A. J. Resistance to Antibiotics: Are We in the Post-Antibiotic Era? Arch. Med. Res. 2005;36(6):697–705. doi: 10.1016/j.arcmed.2005.06.009. - DOI - PubMed
    1. Huh A. J. Kwon Y. J. “Nanoantibiotics”: A new paradigm for treating infectious diseases using nanomaterials in the antibiotics resistant era. J. Controlled Release. 2011;156(2):128–145. doi: 10.1016/j.jconrel.2011.07.002. - DOI - PubMed
    1. Sondi I. Salopek-Sondi B. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J. Colloid Interface Sci. 2004;275(1):177–182. doi: 10.1016/j.jcis.2004.02.012. - DOI - PubMed
    1. Rastogi S. K. Rutledge V. J. Gibson C. Newcombe D. A. Branen J. R. Branen A. L. Ag colloids and Ag clusters over EDAPTMS-coated silica nanoparticles: synthesis, characterization, and antibacterial activity against Escherichia coli. Nanomedicine. 2011;7:305–314. doi: 10.1016/j.nano.2010.11.003. - DOI - PMC - PubMed
    1. Zharov V. P. Mercer K. E. Galitovskaya E. N. Smeltzer M. S. Photothermal Nanotherapeutics and Nanodiagnostics for Selective Killing of Bacteria Targeted with Gold Nanoparticles. Biophys. J. 2006;90(2):619–627. doi: 10.1529/biophysj.105.061895. - DOI - PMC - PubMed