Corrigendum: Reconstruction and Analysis of the lncRNA-miRNA-mRNA Network Based on Competitive Endogenous RNA Reveal Functional lncRNAs in Dilated Cardiomyopathy

Abstract

[This corrects the article DOI: 10.3389/fgene.2019.01149.].

Keywords: competitive endogenous RNA; dilated cardiaomypothy; lncRNA-miRNA-; long non-coding RNA; microRNA.

FIGURE 6

Functional study of miR-144-3p in vitro. (A). qRT-PCR analysis of miRNA between cardiomyocytes and fibroblasts (n = 6). (B,C). qRT-PCR analysis of the transfection efficacy of miR-144-3p with agomir/antagomir compared to controls (n = 6). (C–E). Overexpression or downregulation of miR-144-3p did not perform effects on cardiomyocyte cell size and pathological hypertrophic markers (ANP and BNP), as evidence by α-actin/DAPI staining (n = 4) and qRT-PCR analysis (n = 6). (F). Decreased expression of miR-144-3p in cardiac fibrosis model induced by TGFβ (n = 6). (G,H). Forced expression of miR-144-3p attenuated TGFβ-induced cardiac fibroblast proliferation and trans-differentiation, as evidenced by EdU/α-SMA staining (n = 4) and qRT-PCR analysis of α-SMA, Col1a1 and Col3a1 (n = 6) (I,J). inhibition of miR-144-3p deteriorated TGFb-induced cardiac fibroblasts proliferation and trans-differentiation as evidenced by EdU/α-SMA staining (n = 4) and qRT-PCR analysis (n = 6). Scale bar: 50um. *, p < 0.05; **, p < 0.01; ***, p < 0.001.