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. 2022 May 4:15:2419-2426.
doi: 10.2147/IDR.S364397. eCollection 2022.

Mutation of TonB-Dependent Receptor Encoding Gene MCR_0492 Potentially Associates with Macrolides Resistance in Moraxella catarrhalis Isolates

Zhen Zhang  1 , Zhulan Yang  2 , Xiaohong Xiang  3 , Pu Liao  1 , Changchun Niu  1
Affiliations

Mutation of TonB-Dependent Receptor Encoding Gene MCR_0492 Potentially Associates with Macrolides Resistance in Moraxella catarrhalis Isolates

Zhen Zhang et al. Infect Drug Resist. .

Abstract

Introduction: Moraxella catarrhalis, which is an opportunistic pathogen and is one of the three major pathogens of community-acquired pneumonia, causes a variety of infections in clinic. In recent years, the isolation rate of Moraxella catarrhalis has gradually increased. In China, due to the clinical empirical use of antibiotics, the resistance rate of Moraxella catarrhalis isolated from children to β-lactam antibiotics has reached 99%. The non-susceptible rate of Moraxella catarrhalis to macrolide antibiotics has also increased significantly.

Methods: Two isolates of Moraxella catarrhalis (R17123922_R and R18013231_R) were isolated from in-patients and were confirmed to be resistant to macrolide antibiotics using the standard disk diffusion and broth microdilution method recommended by CLSI. Whole-genome sequencing (WGS) analysis was performed in these two resistant strains.

Results: A total of 696 SNVs (single nucleotide variations), and 79 indels (Insertion and Deletion) were found in R17123922_R and R18013231_R. These SNVs and indels were distributed evenly in the genome, and no centralized distribution occurred. Moreover, two isolates did not harbor any previously reported mutations in the 23S rRNA and ribosomal proteins.

Conclusion: A novel indel in the MCR_0492 gene encoding TonB-dependent receptor protein was identified, and we speculated that TonB-dependent protein receptor may play an important role in macrolide resistance of Moraxella catarrhalis.

Keywords: Moraxella catarrhalis; macrolides; mechanism of drug resistance; whole-genome sequencing.

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Conflict of interest statement

The authors report no conflicts of interest in this work.

Figures

Figure 1
Figure 1
MALDI-TOF MS results of three isolates; green curve was S18010071_S, the blue curve was R17123922_R, and the red curve was R18013231_R.
Figure 2
Figure 2
The results of genomic extraction and 16sRNA electrophoresis of three isolates. (A) The results of genomic extraction from three isolates. Lane 1: S18010071_S, Lane 2:R17123922_R, Lane 3:R18013231_R, Lane 4: DNA Marker 10,000. (B) The electrophoretic results of the products amplified by 16srDNA universal primers. Lane 1:S18010071_S; Lane 2:R17123922_R; Lanes 3 and 4: R18013231_R, Lane 5: DNA Marker 10,000.
Figure 3
Figure 3
Sequencing depth distribution of Moraxella catarrhalis.
Figure 4
Figure 4
SNV and indel mutations of Moraxella catarrhalis were analyzed by Venn diagram. (A) Results of SNV analysis between two macrolide-resistant Moraxella catarrhalis. (B) Results of indel analysis between two macrolide-resistant Moraxella catarrhalis.
Figure 5
Figure 5
Distribution of SNV and indel in genomes of two macrolide-resistant isolates.
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