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. 2022 Apr 25:9:853310.
doi: 10.3389/fsurg.2022.853310. eCollection 2022.

Transient Receptor Potential Channel 1 Potentially Serves as a Biomarker Indicating T/TNM Stages and Predicting Long-Term Prognosis in Patients With Renal Cell Carcinoma

Liang Chen  1 , Guang Shan  1 , Minghuan Ge  1 , Huijun Qian  1 , Yue Xia  1
Affiliations

Transient Receptor Potential Channel 1 Potentially Serves as a Biomarker Indicating T/TNM Stages and Predicting Long-Term Prognosis in Patients With Renal Cell Carcinoma

Liang Chen et al. Front Surg. .

Abstract

Background: Transient receptor potential channel 1 (TRPC1) regulates the progression of several cancers, but its clinical implication in renal cell carcinoma (RCC) has not been explored yet. This study aimed to investigate the correlation of TRPC1 with clinical characteristics and prognosis in patients with RCC.

Methods: Totally, 177 patients with primary RCC who received surgical resection were retrospectively screened. Their tumor and paired adjacent tissue specimens were retrieved to assess TRPC1 mRNA expression using RT-qPCR and TRPC1 protein expression using immunohistochemistry (IHC).

Results: Both TRPC1 IHC score and TRPC1 mRNA expression were elevated in RCC tissue than in adjacent tissue (both P < 0.001). Meanwhile, both TRPC1 IHC score and TRPC1 mRNA expression in tumor were associated with higher T stage (both P = 0.02) and TNM stage (P = 0.009, P = 0.003, respectively). However, no correlation was found in tumor TRPC1 IHC score or TRPC1 mRNA expression with other tumor properties (all P > 0.05). Besides, the 3-, 5-, and 7-year overall survival (OS) were 81.4, 68.6, and 60.2%, respectively in patients with high tumor TRPC1 protein, while they were 89.3, 82.7, and 76.7%, respectively in patients with low tumor TRPC1 protein. High (vs. low) TRPC1 protein in the tumor was associated with shorter OS (P = 0.017), while high (vs. low) TRPC1 mRNA in the tumor was not correlated with OS (P = 0.144). By the forward stepwise method, TRPC1 protein expression independently predicted poor OS (P = 0.01, hazard ratio = 2.052).

Conclusion: TRPC1 serves as a potential biomarker reflecting tumor features and long-term survival profile in patients with RCC.

Keywords: T stage; TNM stage; overall survival; renal cell carcinoma; transient receptor potential channel 1.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Transient receptor potential channel (TRPC1) expression in patients with renal cell carcinoma (RCC). Samples of TRPC1 immunohistochemistry (IHC) staining in the negative control, adjacent and tumor tissues (A); comparison of TRPC1 IHC score (B) and TRPC1 mRNA expression (C) between adjacent and tumor tissues. TRPC1, transient receptor potential channel 1; IHC, immunohistochemistry; mRNA, messenger RNA; RCC, renal cell carcinoma.
Figure 2
Figure 2
Correlation of TRPC1 expression with tumor features in patients with RCC. Correlation of TRPC1 protein expression with tumor location (A), pathological grade (B), tumor size (C), T stage (D), N stage (E), and tumor-node-metastasis (TNM) stage (F); association of TRPC1 mRNA expression with tumor location (G), pathological grade (H), tumor size (I), T stage (J), N stage (K), and TNM stage (L). TRPC1, transient receptor potential channel 1; TNM, tumor-node-metastasis; mRNA, messenger RNA; RCC, renal cell carcinoma.
Figure 3
Figure 3
Association of tumor TRPC1 expression with prognosis in patients with RCC. Association of TRPC1 protein expression (A) and TRPC1 mRNA expression (B) with overall survival (OS). TRPC1, transient receptor potential channel 1; OS, overall survival; mRNA, messenger RNA; RCC, renal cell carcinoma.
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References

    1. Padala SA, Barsouk A, Thandra KC, Saginala K, Mohammed A, Vakiti A, et al. . Epidemiology of renal cell carcinoma. World J Oncol. (2020) 11:79–87. 10.14740/wjon1279 - DOI - PMC - PubMed
    1. Hsieh JJ, Purdue MP, Signoretti S, Swanton C, Albiges L, Schmidinger M, et al. . Renal cell carcinoma. Nat Rev Dis Primers. (2017) 3:17009. 10.1038/nrdp.2017.9 - DOI - PMC - PubMed
    1. Capitanio U, Bensalah K, Bex A, Boorjian SA, Bray F, Coleman J, et al. . Epidemiology of renal cell carcinoma. Eur Urol. (2019) 75:74–84. 10.1016/j.eururo.2018.08.036 - DOI - PMC - PubMed
    1. Arora RD, Limaiem F. Renal Clear Cell Cancer. StatPearls Treasure Island (FL) (2021).
    1. Petejova N, Martinek A. Renal cell carcinoma: review of etiology, pathophysiology and risk factors. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. (2016) 160:183–94. 10.5507/bp.2015.050 - DOI - PubMed

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