Insulin Adsorption onto PE and PVC Tubings

Abstract

We investigate the adsorption of insulin onto PE and PVC materials by using HPLC measurements and computer simulations. We interpret the experiments by calculating the Gibbs free energy profiles during the adsorption process. The values of free energy of adsorption show a good agreement with the experimental measurements. The adsorption of insulin onto the different materials is characterized through the conformational changes with respect to its conformation in water and the interfacial regions, which are described by specific arrangements of polymer chains, water, insulin, and plasticizer molecules.

Keywords: HPLC measurements; adsorption; insulin; microscopic description; plasticized PVC−TOTM surface; polymeric materials; potential of mean force.