Tracking Mycobacterium tuberculosis sequencing samples using unique spikes of random DNA

Abstract

In the Netherlands, local laboratories are involved in the primary diagnosis of tuberculosis. Positive Mycobacterium tuberculosis complex cultures are sent to the National Institute for Public Health and the Environment (RIVM) for species identification, epidemiological typing, and screening for resistance by Whole Genome Sequencing (WGS). Occasional sample-swaps and cross-contaminations are known to occur in the diagnostic procedures. Such errors may lead to incorrect diagnoses resulting in the unnecessary or sub-optimal treatment of patients. Internal controls throughout the process ideally allow the early detection of such mistakes.

Keywords: Cross-contamination; Mycobacterium tuberculosis; Next generation sequencing; Sample-swap; Spike; Whole genome sequencing.