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. 2022 Jun;48(3):785-804.
doi: 10.1007/s10695-022-01081-5. Epub 2022 May 13.

Enzymological, histological, and serum biomarker responses of snubnose pompano on complete replacement of fishmeal using cottonseed meal supplemented with lysine and methionine in the diet

Dhanasekaran Linga Prabu  1 , Pananghat Vijayagopal  2 , Sanal Ebeneezar  2 , Chellappa Kalidas  2 , Palsamy Rameshkumar  2 , Eldho Varghese  2 , Bose Ramar Muniswaran  2
Affiliations

Enzymological, histological, and serum biomarker responses of snubnose pompano on complete replacement of fishmeal using cottonseed meal supplemented with lysine and methionine in the diet

Dhanasekaran Linga Prabu et al. Fish Physiol Biochem. 2022 Jun.

Abstract

In a feeding experiment, cottonseed meal (CSM) was used to replace fishmeal (FM) in the diet of snubnose pompano, Trachinotus blochii, supplemented with lysine and methionine to assess the growth, nutritive profile, hematological, histological, and stress biomarker response. Experimental fishes were randomly stocked in five treatments each with triplicates. Five isonitrogenous and isolipidic diets with graded level of CSM (0, 8.7, 17.4, 26.0, and 34.7%) as replacement for FM protein (0, 25, 50, 75, and 100%) were formulated and fed to respective treatments. Comparison between various parameters among the treatments was made using orthogonal polynomial contrasts to indicate the statistical significance. Higher alkaline phosphatase, acid phosphatase, lactate dehydrogenase, malate dehydrogenase, aspartate, and alanine aminotransferase activities were observed in 0CSM group and followed by 100CSM group as higher inclusion level of CSM with higher free gossypol content did not affect the metabolic enzyme activities. The maximum muscular free gossypol accretion of 1.28 mg kg-1 (on wet basis) was recorded in 100CSM group which was very well below the critical limit set by FDA. As a conclusion, fishmeal can be completely replaced using cottonseed meal in the diet of pompano without adverse effect on growth, metabolism, and general health.

Keywords: Fishmeal replacement; Free gossypol accretion; Lysine deficiency; Sustainable resource; Trachinotus blochii; Unconventional feed ingredient.

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References

    1. Agbo NW, Madalla N, Jauncey K (2015) Mixtures of oilseed meals as dietary protein sources in diets of juvenile Nile tilapia (Oreochromis niloticus L.). J Sci Technol Ghana 35:11–24 - DOI
    1. Ahmed M, Liang H, Kasiya HC, Ji K, Ge X, Ren M, Liu B, Zhu X, Sun A (2019) Complete replacement of fish meal by plant protein ingredients with dietary essential amino acids supplementation for juvenile blunt snout bream (Megalobrama amblycephala). Aquac Nutr 25:205–214. https://doi.org/10.1111/anu.12844 - DOI
    1. Ahmed I, Reshi QM, Fazio F (2020) The influence of the endogenous and exogenous factors on hematological parameters in different fish species: a review. Aquac Int 10:1–31. https://doi.org/10.1007/s10499-019-00501-3 - DOI
    1. Anderson AD, Alam MS, Watanabe WO, Carroll PM, Wedegaertner TC, Dowd MK (2016) Full replacement of menhaden fish meal protein by low-gossypol cottonseed flour protein in the diet of juvenile black sea bass Centropristis striata. Aquaculture 464:618–628. https://doi.org/10.1016/j.aquaculture.2016.08.006 - DOI
    1. AOAC (1995) Official methods of analysis of AOAC International, vol 1, 16th edn. Association of Official Analytical Chemists, Arlington, pp 1230

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