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. 2022 Apr 26:9:877871.
doi: 10.3389/fnut.2022.877871. eCollection 2022.

Structure and Bioactivities of a Novel Polysaccharide Extracted From Dendrobium huoshanense by Subcritical Water

Li Wang  1   2 , Yi-Gui Mao  1 , Xiang Zeng  1 , Na Liu  1 , Chao-Fei Niu  2 , Xin-Xin Li  2 , Bing-Ji Ma  2 , Lan-Ping Guo  3 , Xiao-Long Yang  1
Affiliations

Structure and Bioactivities of a Novel Polysaccharide Extracted From Dendrobium huoshanense by Subcritical Water

Li Wang et al. Front Nutr. .

Abstract

In this study, the polysaccharide was extracted by subcritical water from Dendrobium huoshanense. A novel polysaccharide (DHPs-1) was obtained through several purification steps and its structure and bioactivity were investigated. Structural analysis indicated that the weight-average molecular weight of DHPs-1 was 5.0 × 104 Da and it was mainly composed of glucose (65.04%), mannose (14.23%), galactose (8.17%), galacturonic acid (6.41%), rhamnose (2.34%), and xylose (1.25%). 1,4-Glcp, and 1,4,6-Galp were existed in the backbone of DHPs-1. The residues of 1,3,4-Galp, 1,4-Manp, 1,4-Galp, and 1,3,4,6-Galp could be in the backbone or the side chains with the non-reducing terminal of α-Manp. Bioactivity tests indicated that DHPs-1 had immunomodulatory activity in that it significantly enhanced transcript levels of cytokines [Tumor necrosis factor-α (TNF-α), Interleukin-6 (IL-6), Interleukin-1β (IL-1β), and Interleukin-10 (IL-10)]. DPPH and hydroxyl radical scavenging tests showed that it had good antioxidant activity. These results reveal that DHPs-1 could be developed as a safe immunomodulatory agent and antioxidant for pharmacological or functional food applications.

Keywords: Dendrobium huoshanense; bioactivities; polysaccharide; structure; subcritical water.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Elution curve of DHPs-1 on DEAE-52 (A); FT-IR spectrum of DHPs-1 (B).
Figure 2
Figure 2
The spectra of DHPs-1: (A) 1H; (B) 13C; (C) HSQC; (D) COSY; (E) HMBC; (F) ROESY.
Figure 3
Figure 3
SEM images of DHPs-1 (A1, A2: different magnification of the same region; B1, B2: different magnification of the same region; A1, B1, 800×; A2 B2, 1,000×).
Figure 4
Figure 4
TG, DTG, and DSC curves of DHPs-1.
Figure 5
Figure 5
Antioxidant activity of DPHs-1. (A) DPPH radical scavenging assay; (B) Hydroxyl radical scavenging activity.
Figure 6
Figure 6
Effect of DHPs-1 on the viability of RAW264.7 cells.
Figure 7
Figure 7
Effects of DHPs-1 on the expression of cytokine genes in RAW264.7 cells (A–D): Expression of TNF-α (A), IL-1β (B), IL-6 (C), and IL-10 (D) mRNA in RAW264.7 cells treated with DHPs-1 or LPS groups for 12 h. Significant differences from control group are denoted by *p < 005, **p < 0.01, ***p < 0.001.
See this image and copyright information in PMC

References

    1. Pan L, Li X, Wang M, Zha X, Yang X, Liu Z, et al. . Comparison of hypoglycemic and antioxidative effects of polysaccharides from four different Dendrobium species. Int J Biol Macromol. (2014) 64:420–7. 10.1016/j.ijbiomac.2013.12.024 - DOI - PubMed
    1. Hsieh YSY, Chien C, Liao SKS, Liao SF, Hung WT, Yang WB, et al. . Structure and bioactivity of the polysaccharides in medicinal plant Dendrobium huoshanense. Bioorgan Med Chem. (2008) 16:6054–68. 10.1016/j.bmc.2008.04.042 - DOI - PubMed
    1. Schepetkin IA, Quinn MT. Botanical polysaccharides: macrophage immunomodulation and therapeutic potential. Int Immunopharmacol. (2006) 6:317–33. 10.1016/j.intimp.2005.10.005 - DOI - PubMed
    1. Wu M, Feng H, Song J, Chen L, Xu Z, Xia W, et al. . Structural elucidation and immunomodulatory activity of a neutral polysaccharide from the Kushui Rose (Rosa setate × Rosa rugosa) waste. Carbohydr Polym. (2020) 232:115804. 10.1016/j.carbpol.2019.115804 - DOI - PubMed
    1. Ji X, Guo J, Ding D, Gao J, Hao L, Guo X, et al. . Structural characterization and antioxidant activity of a novel high-molecular-weight polysaccharide from Ziziphus Jujuba cv. Muzao. J Food Meas Charact. (2022). 10.1007/s11694-022-01288-3 - DOI

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