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. 1987:66:91-9.

S-phase cell detection with a monoclonal antibody

H G Miltenburger, G Sachse, M Schliermann
  • PMID: 3556275

S-phase cell detection with a monoclonal antibody

H G Miltenburger et al. Dev Biol Stand. 1987.

Abstract

The use of a newly developed monoclonal antibody against Bromodeoxyuridine is demonstrated in a study with V79 Chinese hamster cells. It can replace radioactive material in cell cycle analysis studies in the way that BrdU incorporated into the DNA during S-phase is detected by immunofluorescence. By this technique the proportion of actively synthesizing S-phase cells can be determined. The proportion of such cells is a good measure with regard to the quality of a culture and/or medium in question. The method is fast and offers within 3-5 hours the possibility to analyse growth conditions in monolayers and suspension cultures.

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