Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Apr 28;11(9):1287.
doi: 10.3390/foods11091287.

Rapid Detection of Carbendazim Residue in Apple Using Surface-Enhanced Raman Scattering and Coupled Chemometric Algorithm

Xiaowei Huang  1   2 , Ning Zhang  1   2 , Zhihua Li  1   2 , Jiyong Shi  1   2 , Haroon Elrasheid Tahir  1   2 , Yue Sun  1   2 , Yang Zhang  1   2 , Xinai Zhang  1   2 , Melvin Holmes  3   4 , Xiaobo Zou  1   2   4   5
Affiliations

Rapid Detection of Carbendazim Residue in Apple Using Surface-Enhanced Raman Scattering and Coupled Chemometric Algorithm

Xiaowei Huang et al. Foods. .

Abstract

In order to achieve rapid and precise quantification detection of carbendazim residues, surface-enhanced Raman spectroscopy (SERS) combined with variable selected regression methods were developed. A higher sensitivity and greater density of "hot spots" in three-dimensional (3D) SERS substrates based on silver nanoparticles compound polyacrylonitrile (Ag-NPs @PAN) nanohump arrays were fabricated to capture and amplify the SERS signal of carbendazim. Four Raman spectral variable selection regression models were established and comparatively assessed. The results showed that the bootstrapping soft shrinkage-partial least squares (BOSS-PLS) method achieved the best predictive capacity after variable selection, and the final BOSS-PLS model has the correlation coefficient (RP) of 0.992. Then, this method used to detect the carbendazim residue in apple samples; the recoveries were 86~116%, and relative standard deviation (RSD) is less than 10%. The 3D SERS substrates combined with the BOSS-PLS algorithm can deliver a simple and accurate method for trace detection of carbendazim residues in apples.

Keywords: Ag-NPs @PAN-nanohump arrays; apple; bootstrapping soft shrinkage-partial least squares (BOSS-PLS); carbendazim; surface-enhanced Raman spectroscopy (SERS).

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Figure 1
Figure 1
(a) Scheme of the fabrication of the SERS chip; (b) schematic representation of the detection of carbendazim residues in apple; (c) schematic illustration the chemometric algorithms.
Figure 2
Figure 2
(a) SEM images of Ag-NPs @PAN-nano-hemisphere film. (b) The existence of Ag in the EDS pattern confirmed the elemental composition of Ag-NPs @PAN-nano-hemisphere film.
Figure 3
Figure 3
Raman spectrum and SERS spectrum of carbendazim (a); raw SERS spectra with seven different concentrations of carbendazim (b); 252 SERS spectra of carbendazim (c); and the MSC preprocessed spectra (d).
Figure 4
Figure 4
(a) SERS spectra and (b) peak intensity at 1225 cm−1 of carbendazim (0.01 mg/L) and other five pesticides (10 mg/L) in ethanol/water (1:1, v/v) solution. The spectra from a to g are carbendazim, isocarbophos, captan, parathion-methyl, thiram, chlorpyrifos, and blank samples.
See this image and copyright information in PMC

References

    1. Huang Y.P., Lu R.F., Chen K.J. Detection of internal defect of apples by a multichannel Vis/NIR spectroscopic system. Postharvest Biol. Technol. 2020;161:111065. doi: 10.1016/j.postharvbio.2019.111065. - DOI
    1. El Hawari K., Mokh S., Al Iskandarani M., Halloum W., Jaber F. Pesticide residues in Lebanese apples and health risk assessment. Food Addit. Contam. Part B-Surveill. 2019;12:81–89. doi: 10.1080/19393210.2018.1564370. - DOI - PubMed
    1. Chuang S., Yang H., Wang X., Xue C., Jiang J., Hong Q. Potential effects of Rhodococcus qingshengii strain djl-6 on the bioremediation of carbendazim-contaminated soil and the assembly of its microbiome. J. Hazard. Mater. 2021;414:125496. doi: 10.1016/j.jhazmat.2021.125496. - DOI - PubMed
    1. Wang Y.R., Lian S., Dong X.L., Wang C.X., Li B.H., Li P.L. Analysis of the dissipation kinetics of thiophanate-methyl and its metabolite carbendazim in apple leaves using a modified QuEChERS-UPLC-MS/MS method. Biomed. Chromatogr. 2019;33:e4394. doi: 10.1002/bmc.4394. - DOI - PubMed
    1. Yao Y., Wen Y., Zhang L., Wang Z., Zhang H., Xu J. Electrochemical recognition and trace-level detection of bactericide carbendazim using carboxylic group functionalized poly(3,4-ethylenedioxythiophene) mimic electrode. Anal. Chim. Acta. 2014;831:38–49. doi: 10.1016/j.aca.2014.04.059. - DOI - PubMed

LinkOut - more resources