The Effects of Agrimonia pilosa Ledeb, Anemone chinensis Bunge, and Smilax glabra Roxb on Broiler Performance, Nutrient Digestibility, and Gastrointestinal Tract Microorganisms

Abstract

Poultry farming is growing globally, particularly in developing countries, to meet the demands of growing populations for poultry meat and eggs. This is likely to lead to an increase in the use of antibiotics in poultry feed, thus contributing to the development and spread of antibiotic resistance which, poses a serious threat to human and animal health worldwide. One way of reducing this threat is to reduce the use of antibiotics in poultry production by finding effective and sustainable antibiotic alternatives that can be used to support poultry health and productivity. Therefore, this study evaluates the incorporation of three medicinal plants, Anemone chinensis Bunge, Smilax glabra Roxb, and Agrimonia pilosa Ledeb, in poultry feed on production performance, nutrient digestibility, and bacteria in the chicken caecum in a 35-day performance trial with 420-day-old male Ross 308 broilers. Groups of randomly selected chicks received one of six dietary treatments. These included five experimental diets of reduced nutrient specifications as a negative control (NC); with amoxicillin as a positive antibiotic control (PC1); with A. pilosa Ledeb (NC1); with A. chinensis Bunge (NC2); and with S. glabra Roxb (NC3). One other positive control diet contained the recommended nutrient specification (PC2). Weight gain and feed intake were measured weekly and used to calculate the feed conversion ratio as performance parameters. Bacteria were enumerated from chicken caecum using a traditional plating method and selective agar. S. glabra Roxb and A. chinensis Bunge showed comparable effects to amoxicillin with significantly increased weight gain in birds offered these diets, compared to those offered the negative control from days 0 to 35 (p < 0.001). S. glabra Roxb exhibited effects similar to the amoxicillin control group with an improved feed conversion ratio (p < 0.001). In addition, S. glabra Roxb decreased numbers of E. coli and Campylobacter spp. on days 21 (p < 0.05) and 35 (p < 0.01) and increased numbers of lactic acid bacteria comparable to the antibiotic group on days 14 (p < 0.001) and 35 (p < 0.01). The findings of this in vivo trial highlight the potential of S. glabra Roxb and A. chinensis Bunge as beneficial feed material to promote poultry health and productivity in the absence of antibiotics.

Keywords: Agrimonia pilosa Ledeb; Anemone chinensis Bunge; and Smilax glabra Roxb; broiler chickens; lactic acid bacteria; performance; plants.

Figure 1

The effect of the plant materials on the bacterial load of Campylobacter spp. in the caecum from 7 to 35 days of age. Means without common superscripts (^ab) indicate the significant differences (*, p < 0.05)between the treatments. PC1 = positive control 1 (NC+40mg/kg amoxicillin); PC2 = positive control 2 (recommended nutrient specification); NC = negative control (reduced nutrient specification); NC1 = NC + herb of A. pilosa Ledeb; NC2 = NC + herb of A. chinensis Bunge; and NC3 = NC + tuber of S. glabra Roxb.

Figure 2

The effect of the plant materials on the bacterial load of E. coli in the caecum from 7 to 35 days of age. Means without common superscripts (^ab) indicate the significant differences (*, p < 0.05) between the treatments. PC1 = positive control 1 (NC + 40mg/kg amoxicillin); PC2 = positive control 2 (recommended nutrient specification); NC = negative control (reduced nutrient specification); NC1 = NC + herb of A. pilosa Ledeb; NC2 = NC + herb of A. chinensis Bunge; and NC3 = NC + tuber of S. glabra Roxb.