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. 2022 Apr 20;27(9):2634.
doi: 10.3390/molecules27092634.

Quality Assessment of Artemisia rupestris L. Using Quantitative Analysis of Multi-Components by Single Marker and Fingerprint Analysis

Xueqin Cao  1   2   3 , Muchun Li  1   2   3 , Liuchun Ma  3 , Miaomiao Wang  1   2   3 , Xueling Hou  1 , Maitinuer Maiwulanjiang  1
Affiliations

Quality Assessment of Artemisia rupestris L. Using Quantitative Analysis of Multi-Components by Single Marker and Fingerprint Analysis

Xueqin Cao et al. Molecules. .

Abstract

The chromatographic fingerprint of 14 batches of Artemisia rupestris L. samples were established in this study. The constituents of ten components in Artemisia rupestris L. were determined using quantitative analysis of multi-components by single marker (QAMS) and the external standard method (ESM). Due to their stability and accessibility, chlorogenic acid and linarin were used as references to calculate the relative correction factors (RCFs) of apigenin-C-6,8-pentoside-hexoside, apigenin-C-6,8-di-pentoside, luteolin, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, chrysosplenetin B, and sbsinthin, based on high-performance liquid chromatography (HPLC). The value calculated by QAMS was consistent with that of the ESM, and the reproducibility of RCFs was found to be reliable. In conclusion, simultaneous determination of the ten components by the QAMS method and chromatographic fingerprint analysis were feasible and accurate in evaluating the quality of Artemisia rupestris L. and can be used as reference in traditional Chinese medicine quality control.

Keywords: Artemisia rupestris L.; fingerprint analysis; high-performance liquid chromatography; quality control; quantitative analysis of multi-components by single marker.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
HPLC chromatograms of the mixed standards and A. rupestris sample. (a) the mixed standards; (b) A. rupestris sample. 1—Chlorogenic acid; 2—Apigenin-C-6,8-pentoside-hexoside; 3—Apigenin-C-6,8-di-pentoside; 4—Luteolin; 5—3,4-Dicaffeoylquinic acid; 6—3,5-Dicaffeoylquinic acid; 7—4,5-Dicaffeoylquinic acid; 8—Linarin; 9—Chrysosplenetin B; 10—Sbsinthin.
Figure 2
Figure 2
The fingerprints of A. rupestris samples. 1—Chlorogenic acid; 2—Apigenin-C-6,8-pentoside-hexoside; 3—Apigenin-C-6,8-di-pentoside; 4—Luteolin; 5—3,4-Dicaffeoylquinic acid; 6—3,5-Dicaffeoylquinic acid; 7—4,5-Dicaffeoylquinic acid; 8—Linarin; 9—Chrysosplenetin B; 10—Sbsinthin.
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