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. 2022 May 1;11(9):2544.
doi: 10.3390/jcm11092544.

Genomic Mutations of BK Polyomavirus in Patients after Kidney Transplantation: A Cross-Sectional Study in Vietnam

Truong Quy Kien  1 , Pham Quoc Toan  1 , Phan Ba Nghia  1 , Diem Thi Van  1 , Nguyen Van Duc  1 , Do Manh Ha  1 , Nguyen Thi Thuy Dung  1 , Nguyen Thi Thu Ha  1 , Le Thi Bao Quyen  2 , Hoang Trung Vinh  1 , Bui Van Manh  3 , Hoang Xuan Su  4 , Tran Viet Tien  5 , Le Viet Thang  1 , Lionel Rostaing  6
Affiliations

Genomic Mutations of BK Polyomavirus in Patients after Kidney Transplantation: A Cross-Sectional Study in Vietnam

Truong Quy Kien et al. J Clin Med. .

Abstract

Objectives: The purpose of this study was to identify the SNP sites and determine the BKV genotype circulating in kidney-transplant Vietnamese recipients based on the VP1 gene region.

Methods: 344 samples were collected from post-kidney-transplant recipients at the 103 Vietnam Military Hospital to investigate the number of BKV infections. Positive samples with a sufficient virus concentration were analyzed by nested PCR in the VP1 region, sequencing detected genotyping and single-nucleotide polymorphism.

Results: BKV infection was determined in 214 patients (62.2%), of whom 11 (5.1%) were diagnosed with BKV-associated nephropathy. Among the 90 BKV-I strains sequenced, 89 (98.88%) were strains of I/b-1 and 1 (1.12%) was strain I/b-2. The 60 BKV-IV strains had a greater diversity of subgroups, including 40% IV/a-1, 1.66% IV/a-2, 56.68% IV/c-1, and 1.16% IV/c-2. Additionally, of 11 cases diagnosed with BKVN, seven belonged to subgroup I/b-1 (63.6%) and four to subgroup IV/c-1 (36.4%). Moreover, 22 specific SNPs that were genotype I or IV were determined in this Vietnamese population. Specifically, at position 1745, for the Vietnamese BKV-IV strains, the SNP position (A→G) appeared in 57/60 samples (95%). This causes transformation of the amino acid N→S. This SNP site can enable detection of genotype IV in Vietnam. It represents a unique evolution pattern and mutation that has not been found in other international strains.

Conclusion: The BKV-I genotype was more common than BKV-IV; however, mutations that occur on the VP1 typing region of BKV-IV strains were more frequent than in BKV-I strains.

Keywords: BK polyomavirus; VP1 region; Vietnam; amino acid; genotype; kidney transplantation; single nucleotide polymorphism.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
NJ phylogenetic tree clustering of 90 BKV-I polyomavirus sequences to determine subgroups.
Figure 2
Figure 2
NJ phylogenetic tree clustering 60 BKV-IV polyomavirus sequences to determine subgroups.
See this image and copyright information in PMC

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