Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 May 14;255(6):125.
doi: 10.1007/s00425-022-03893-4.

N6-Methyladenosine dynamic changes and differential methylation in wheat grain development

Wenxiang Li  1 , Yi Yu  1 , Xuanrong Chen  1 , Qian Fang  1 , Anqi Yang  1 , Xinyu Chen  1 , Lei Wu  1 , Chengyu Wang  1   2 , Dechuan Wu  1 , Sihong Ye  3 , Dexiang Wu  4 , Genlou Sun  5
Affiliations

N6-Methyladenosine dynamic changes and differential methylation in wheat grain development

Wenxiang Li et al. Planta. .

Abstract

More methylation changes occur in late interval than in early interval of wheat seed development with protein and the starch synthesis-related pathway enriched in the later stages. Wheat seed development is a critical process to determining wheat yield and quality, which is controlled by genetics, epigenetics and environments. The N6-methyladenosine (m6A) modification is a reversible and dynamic process and plays regulatory role in plant development and stress responses. To better understand the role of m6A in wheat grain development, we characterized the m6A modification at 10 day post-anthesis (DPA), 20 DPA and 30 DPA in wheat grain development. m6A-seq identified 30,615, 30,326, 27,676 high confidence m6A peaks from the 10DPA, 20DPA, and 30DPA, respectively, and enriched at 3'UTR. There were 29,964, 29,542 and 26,834 unique peaks identified in AN0942_10d, AN0942_20d and AN0942_30d. One hundred and forty-two genes were methylated by m6A throughout seed development, 940 genes methylated in early grain development (AN0942_20d vs AN0942_10d), 1542 genes in late grain development (AN0942_30d vs AN0942_20d), and 1190 genes between early and late development stage (AN0942_30d vs AN0942_10d). KEGG enrichment analysis found that protein-related pathways and the starch synthesis-related pathway were significantly enriched in the later stages of seed development. Our results provide novel knowledge on m6A dynamic changes and its roles in wheat grain development.

Keywords: Differential methylation; Genome; Grain development; Regulation; Triticum aestivum; m6A methylation.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Anders S, Huber W (2012) Differential expression of RNA-Seq data at the gene level–the DESeq package. European Molecular Biology Laboratory (EMBL), Heidelberg
    1. Bailey TL (2011) DREME: motif discovery in transcription factor ChIP-seq data. Bioinformatics 27:1653–1659 - PubMed - PMC - DOI
    1. Bailey TL, Boden M, Buske FA, Frith M, Grant CE, Clementi L, Ren J, Li WW, Noble WS (2009) MEME SUITE: tools for motif discovery and searching. Nucleic Acids Res 37:202–208 - DOI
    1. Barneix AJ (2007) Physiology and biochemistry of source-regulated protein accumulation in the wheat grain. J Plant Physiol 164:581–590 - PubMed - DOI
    1. Bodi Z, Zhong S, Mehra S, Song J, Graham N, Li H, May S, Fray RG (2012) Adenosine methylation in Arabidopsis mRNA is associated with the 3’ end and reduced levels cause developmental defects. Front Plant Sci 3:48 - PubMed - PMC - DOI

LinkOut - more resources