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. 2022 May 14;24(1):109.
doi: 10.1186/s13075-022-02796-9.

Increased frequency of IgD-CD27hiCD38hi B cells and its association with the renal involvement in ANCA-associated vasculitis

Chen Wang  1   2   3 , Zhi-Ying Li  4   5   6 , Yan Gong  7 , Ran You  7 , Ming-Hui, Zhao  4   5   6   8 , Min Chen  4   5   6
Affiliations

Increased frequency of IgD-CD27hiCD38hi B cells and its association with the renal involvement in ANCA-associated vasculitis

Chen Wang et al. Arthritis Res Ther. .

Abstract

Background: B cells have been highlighted in the pathogenesis of antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) by the identification of activated B cells in granulomatous lesions and the efficacy of B cell depletion in treatment of AAV patients in the current study; we aimed to investigate the frequency of a specific B cell subset, IgD-CD27hiCD38hi B cells in AAV patients, and its association with the disease severity of AAV.

Methods: Blood samples of patients with AAV in active stage and in remission were collected. The frequency of IgD-CD27hiCD38hi B cells was detected by flow cytometry, and its correlation with clinicopathological parameters was analyzed.

Results: Our results showed a significant increase of circulating IgD-CD27hiCD38hi B cells in AAV patients in active stage compared with patients in remission and healthy donors, and the frequency of IgD-CD27hiCD38hi B cells correlated with the severity of renal involvement, including serum creatinine, estimated glomerular filtration rate, and percentages of total crescents in renal biopsies.

Conclusions: The results indicated that IgD-CD27hiCD38hi B cells could reflect disease severity of renal involvement in AAV.

Keywords: Antineutrophil cytoplasmic antibody; B cells; Renal involvement; Vasculitis.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
A representative gating example of sequential blood sample from an AAV patient. A The distribution of IgD-CD27hiCD38hi B cells in the patient in active stage. B the distribution of IgD-CD27hiCD38hi B cells in the patient in remission
Fig. 2
Fig. 2
Increased frequency of IgD-CD27hiCD38hi B cells in patients with active AAV. A The frequency of IgD-CD27hiCD38hi B cells among CD19+ B cells was significantly increased in the AAV patients in active stage than those in both AAV patients in remission and healthy donors. B The frequency of IgD-CD27hiCD38hi B cells among CD45+ leukocytes was significantly increased in the AAV patients in active stage than those in both AAV patients in remission and healthy donors. C The frequency of IgD-CD27hiCD38hi B cells among CD19+ B cells was significantly increased in active stage than that in remission in 8 AAV patients with sequential blood samples of both active stage and remission. D The frequency of IgD-CD27hiCD38hi B cells among CD45+ leukocytes was significantly increased in active stage than that in remission in 8 AAV patients with sequential blood samples of both active stage and remission
Fig. 3
Fig. 3
Association between frequency of IgD-CD27hiCD38hi B cells and severity of renal involvement in patients with active AAV. A The frequency of IgD-CD27hiCD38hi B cells correlated with Scr. B The frequency of IgD-CD27hiCD38hi B cells correlated with eGFR. C The percentages of total crescents in renal biopsies were significantly higher in patients with ≥7.29% IgD-CD27hiCD38hi B cells frequency than patients with <7.29% IgD-CD27hiCD38hi B cells frequency
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