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. 2022:2503:95-104.
doi: 10.1007/978-1-0716-2333-6_6.

Laboratory Diagnosis and Quantification of African Swine Fever Virus Using Real-Time Polymerase Chain Reaction

Christopher L Netherton  1 , Lynnette C Goatley  1 , John Flannery  2 , Martin Ashby  2 , Carrie Batten  3
Affiliations

Laboratory Diagnosis and Quantification of African Swine Fever Virus Using Real-Time Polymerase Chain Reaction

Christopher L Netherton et al. Methods Mol Biol. 2022.

Abstract

Real-time polymerase chain reaction (PCR) for the detection of African swine fever virus (ASFV) is the tool of choice for the diagnostic laboratory and is a robust and easily scalable method for the researcher analyzing viral replication both in vitro and in vivo. In this chapter, we describe protocols for both quantitative real-time polymerase chain reactions (qPCR) and non-quantitative real-time polymerase chain reactions (real-time PCR) for the detection of African swine fever virus genome in a range of samples.

Keywords: African swine fever; Diagnosis; Real-time PCR; TaqMan®; qPCR.

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References

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