African Swine Fever Virus Plaque Assay and Disinfectant Testing
- PMID: 35575896
- DOI: 10.1007/978-1-0716-2333-6_14
African Swine Fever Virus Plaque Assay and Disinfectant Testing
Abstract
Quantifying the titer of African swine fever virus is critical for disease control, viral infection studies, and disinfectant efficacy tests. Techniques such as real-time PCR and virus isolation provide an understanding as to whether viral genome is present or gives a qualitative assessment of live viral presence in a sample respectively, but neither provide a quantitative measurement of live virus. Here we describe a plaque assay for the titration of a Vero-adapted African swine fever virus strain (BA71V) and describe how to apply this method to determine disinfectant efficacy.
Keywords: African swine fever virus; Disinfectant; Plaque assay.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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