Phytochemical Studies, Antioxidant Potential, and Identification of Bioactive Compounds Using GC-MS of the Ethanolic Extract of Luffa cylindrica (L.) Fruit

Abstract

Luffa cylindrica (L.) is a medicinal plant associated with Cucurbitaceae family which is also known as loofah/sponge gourd, comprising a series of phytochemicals such as chlorophylls, carotenoids, oleanolic acid, saponin, and triterpenoids. The study was carried out to investigate and characterize the bioactive components of ethanolic extract of L. cylindrica. Whole fruit of L. cylindrica was collected, shade dried, pulverized, and extracted successively with ethanol by Soxhlet percolation technique. The crude extracts were later exposed to gas chromatography-mass spectrometry analysis. The profile of the extracts was analyzed for a wide range of secondary metabolites and characterized spectroscopically. A total of 18 components were identified in the ethanolic extract respectively. Prevailing pharmacologically active compounds benzaldehyde, 2-hydroxy-4-methyl-, 4-acetoxy-2-azetidinone, N-decanoic acid, oxirane,2-butyl-3-methyl-, cis, and 3,4-furandiol, tetrahydro-, cis- were present. The extracted compounds were articulated by comparing their retention time and peak area besides the interpretation of mass spectra. Thus, the current study reveals the presence of promising, bioactive components which in turn provides a strength to explore biological activity. In silico molecular docking could be performed for Alzheimer receptors and studied for its activity. Nevertheless, additional studies are required to carry out its bioactivity exploration and toxicity profile.

Keywords: Alzheimer; DPPH; GC–MS; Luffa cylindrica (L.); Soxhlet.